Schwan T G, Simpson W J
Department of Health and Human Services, National Institutes of Health, National Institute of Allergy and Infectious Diseases, Hamilton, Montana.
Scand J Infect Dis Suppl. 1991;77:94-101.
The immunologic reactivity of some antigens of low passage Borrelia burgdorferi varied with the temperature at which it was cultivated in vitro, with long-term cultivation in vitro, and following passage in white-footed mice, Peromyscus leucopus. The low passaged uncloned strain Sh-2-82 had either more antigens or antigens which were more immunoreactive when grown at various temperatures from 28-39 degrees C. The influence of temperatures was less evident, however with a higher passage of the same strain. Immune sera from white-footed mice experimentally infected with the spirochete were more reactive with antigens of the inoculum than with antigens of spirochetes reisolated later. The antigenicity of the Lyme borreliosis spirochete is becoming more complex and this may relate to the complex life cycle that includes tick and mammalian hosts.
低传代伯氏疏螺旋体某些抗原的免疫反应性会因体外培养温度、体外长期培养以及在白足鼠（白足鼠属）体内传代而发生变化。低传代未克隆菌株Sh - 2 - 82在28至39摄氏度的不同温度下生长时，要么具有更多抗原，要么抗原的免疫反应性更强。然而，对于同一菌株的高传代情况，温度的影响则不太明显。经螺旋体实验感染的白足鼠的免疫血清与接种物的抗原反应性比与后来重新分离的螺旋体的抗原反应性更强。莱姆病螺旋体的抗原性正变得更加复杂，这可能与包括蜱和哺乳动物宿主在内的复杂生命周期有关。
