Lashkov A A, Gabdoulkhakov A G, Shtil A A, Mikhailov A M
A. V. Shubnikov Institute of Crystallography, Russian Academy of Sciences, Leninskiy Prospect 59, 119333 Moscow, Russia.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Jun 1;65(Pt 6):601-3. doi: 10.1107/S1744309109016133. Epub 2009 May 22.
Uridine phosphorylase (UPh; EC 2.4.2.3) catalyzes the phosphorolytic cleavage of the N-glycosidic bond of uridine to form ribose 1-phosphate and uracil. This enzyme also activates pyrimidine-containing drugs, including 5-fluorouracil (5-FU). In order to better understand the mechanism of the enzyme-drug interaction, the complex of Salmonella typhimurium UPh with 5-FU was cocrystallized using the hanging-drop vapour-diffusion method at 294 K. X-ray diffraction data were collected to 2.2 A resolution. Analysis of these data revealed that the crystal belonged to space group C2, with unit-cell parameters a = 158.26, b = 93.04, c = 149.87 A, alpha = gamma = 90, beta = 90.65 degrees . The solvent content was 45.85% assuming the presence of six hexameric molecules of the complex in the unit cell.
尿苷磷酸化酶（UPh；EC 2.4.2.3）催化尿苷的N-糖苷键进行磷酸解裂解，形成1-磷酸核糖和尿嘧啶。该酶还能激活含嘧啶的药物，包括5-氟尿嘧啶（5-FU）。为了更好地理解酶与药物相互作用的机制，采用悬滴气相扩散法在294 K下使鼠伤寒沙门氏菌UPh与5-FU的复合物共结晶。收集了分辨率为2.2 Å的X射线衍射数据。对这些数据的分析表明，该晶体属于空间群C2，晶胞参数为a = 158.26、b = 93.04、c = 149.87 Å，α = γ = 90°，β = 90.65°。假设晶胞中存在六个复合物的六聚体分子，则溶剂含量为45.85%。
