Zhang Shuhong, Li Jianfeng, Jiang Ying, Xu Yijun, Qin Chengyong
Department of Gastroenterology, Shandong Provincial Hospital Affiliated to Shandong University324 Jingwu Weiqi Road, Jinan 250021, PR China.
J Exp Clin Cancer Res. 2009 May 29;28(1):71. doi: 10.1186/1756-9966-28-71.
Hepatocellular carcinoma (HCC) is a lethal malignancy with high rate of metastasis and poor prognosis. There are no effective managements to block metastasis of HCC. Programmed cell death 4 (PDCD4) is found to be a tumor transformation suppressor. Among investigations on effects of PDCD4, little is about the metastatic potentials of HCC cells. This study was to investigate the role of PDCD4 on metastatic potential of human HCC cells.
We examined the expression of PDCD4 in three HCC cell lines with different metastatic potentials, MHCC-97H (high metastatic potential), MHCC-97L (low metastatic potential) and Hep3B (no metastatic potential). A plasmid encoding PDCD4 gene was constructed and then transfected into HCC cells with the lowest PDCD4 expression level. Effects of PDCD4 on cell proliferation, cell apoptosis, gene expression of metastasis tumor antigen 1 (MTA1) and in vitro migration and invasion capacity were assessed after transfection.
Our results showed that the expression level of PDCD4 was inversely correlated to the metastatic potential of HCC cells. After transfection with the PDCD4 gene, HCC cell proliferation rate was significantly decreased, cell apoptosis rate was significantly increased, the expression of MTA1 gene, HCC cell migration and Matrigel invasion were also remarkably inhibited.
PDCD4 expression is inversely correlated to the metastatic potential of HCC cells. PDCD4 can effectively suppress the metastatic potential of HCC cells.
肝细胞癌（HCC）是一种具有高转移率和不良预后的致命恶性肿瘤。目前尚无有效的方法来阻断HCC的转移。程序性细胞死亡4（PDCD4）被发现是一种肿瘤转化抑制因子。在对PDCD4作用的研究中，关于HCC细胞转移潜能的研究较少。本研究旨在探讨PDCD4在人HCC细胞转移潜能中的作用。
我们检测了三种具有不同转移潜能的HCC细胞系，即MHCC-97H（高转移潜能）、MHCC-97L（低转移潜能）和Hep3B（无转移潜能）中PDCD4的表达。构建了编码PDCD4基因的质粒，然后将其转染到PDCD4表达水平最低的HCC细胞中。转染后评估PDCD4对细胞增殖、细胞凋亡、转移肿瘤抗原1（MTA1）基因表达以及体外迁移和侵袭能力的影响。
我们的结果表明，PDCD4的表达水平与HCC细胞的转移潜能呈负相关。用PDCD4基因转染后，HCC细胞增殖率显著降低，细胞凋亡率显著增加，MTA1基因的表达、HCC细胞迁移和基质胶侵袭也受到显著抑制。
PDCD4表达与HCC细胞的转移潜能呈负相关。PDCD4可有效抑制HCC细胞的转移潜能。
