Wong M M, Foskett J K
Division of Cell Biology, Research Institute, Hospital for Sick Children, Toronto, Ontario, Canada.
Science. 1991 Nov 15;254(5034):1014-6. doi: 10.1126/science.1948071.
In acinar cells from rat salivary glands, cholinergic agonists cause oscillations in cytoplasmic free calcium concentration, which then drive oscillations of cell volume that reflect oscillating cell solute content and fluid secretion. By quantitative fluorescence ratio microscopy of an intracellular indicator dye for sodium, it has now been shown that large amplitude oscillations of sodium concentration were associated with the calcium and cell volume oscillations. Both calcium and sodium oscillations were dependent on the continued presence of calcium in the extracellular medium and were abolished by the specific sodium-potassium adenosine triphosphatase inhibitor ouabain. Thus, calcium oscillations in salivary acinar cells, by modulating the activities of ion transport pathways in the plasma membrane, can cause significant oscillations of monovalent ions that may in turn feed back to regulate calcium oscillations and fluid secretion.
在大鼠唾液腺的腺泡细胞中，胆碱能激动剂可引起细胞质游离钙浓度的振荡，进而驱动细胞体积的振荡，这反映了细胞溶质含量和液体分泌的振荡。通过对一种用于检测钠的细胞内指示染料进行定量荧光比率显微镜观察，现已表明钠浓度的大幅度振荡与钙和细胞体积的振荡有关。钙振荡和钠振荡均依赖于细胞外介质中钙的持续存在，并被特异性钠钾腺苷三磷酸酶抑制剂哇巴因所消除。因此，唾液腺泡细胞中的钙振荡通过调节质膜中离子转运途径的活性，可引起单价离子的显著振荡，而单价离子振荡反过来可能反馈调节钙振荡和液体分泌。
