Foskett J K
Division of Cell Biology, Hospital for Sick Children, Toronto, Ontario, Canada.
Am J Physiol. 1990 Dec;259(6 Pt 1):C998-1004. doi: 10.1152/ajpcell.1990.259.6.C998.
Differential interference contrast microscopy and low-light-level digital imaging of the fluorescent chloride indicator dye 6-methyl-1-(3-sulfonatopropyl)quinolinium (SPQ) were performed simultaneously in single mammalian salivary gland acinar cells to examine the relationship between cytoplasmic chloride concentration [( Cl-]i) and cell volume during stimulus-secretion coupling. Agonist stimulation of Cl(-)-driven fluid secretion is associated with rapid, Ca2(+)-dependent changes of cell volume, which are temporally coupled to changes of [Cl-]i. The agonist-induced changes in [Cl-]i, if accompanied by cations and water, quantitatively account for the cell volume changes, demonstrating in a single cell that cell volume is determined by cell solute content. Agonist-induced modulation of cell volume appears to be a consequence of the requirement to develop appropriate ion gradients necessary for vectorial salt (and fluid) transport.
在单个哺乳动物唾液腺腺泡细胞中，同时进行了微分干涉对比显微镜检查和荧光氯化物指示剂染料6-甲基-1-（3-磺丙基）喹啉鎓（SPQ）的低光水平数字成像，以研究刺激-分泌偶联过程中细胞质氯化物浓度[（Cl-）i]与细胞体积之间的关系。氯离子驱动的液体分泌的激动剂刺激与细胞体积的快速、钙依赖变化相关，这些变化在时间上与[Cl-]i的变化相关联。如果激动剂诱导的[Cl-]i变化伴随着阳离子和水，那么从数量上可以解释细胞体积的变化，这在单个细胞中表明细胞体积由细胞溶质含量决定。激动剂诱导的细胞体积调节似乎是形成矢量盐（和液体）运输所需适当离子梯度的结果。
