Wei Haiming, Tian Zhigang, Xu Xiaoqun, Feng Jinbo, Xiao Wei
Institute of Shandong Academy of Basic Medical Sciences, Shandong University Medical School, Jinan 250062, China.
Zhonghua Zhong Liu Za Zhi. 2002 Jan;24(1):34-7.
To study the relation between the expression of transcription factor T-bet/GATA3 and Th1/Th2 type cytokines in peripheral blood mononuclear cells (PBMC) from lung cancer patients and their interference by the traditional Chinese herbal medicine.
The gene expression of Th1/Th2 type cytokine IFN gamma, IL-2, IL-4, IL-6, IL-10, transcription factor T-bet/GATA3 and tumor tissue specific mRNA CEA, CK19 in PBMC from lung cancer patients were detected by reverse transcription-polymerase chain reaction RT-PCR. Meanwhile, the change of IFN gamma, IL-4, T-bet and GATA3 in PBMC before and after being cultured with the traditional Chinese herbal medicine-Astragulus and Tetramethylpyrazine was also observed.
Predominant expression of Th2 type cytokines was detected in 42 lung cancer patients. The positive rates of IL-4, IL-6, IL-10, IFN gamma and IL-2 were 27/42, 24/42, 31/42, 4/42 and 5/42, respectively. But, the positive rates of transcription factor T-bet and GATA3 were 16/42 and 34/42. Moreover, the expression intensity of T-bet was lower in the CEA and CK19 positive patients than the negative ones. On the contrary, the expression intensity of GATA3 was significantly higher in the same patients.
Predominant expression of Th2 type cytokines may be related to lower expression of T-bet or higher expression of GATA3. This condition can be interfered by the traditional Chinese herbal medicine-Astragulus and Tetramethylpyrazine.
研究肺癌患者外周血单个核细胞（PBMC）中转录因子T-bet/GATA3的表达与Th1/Th2型细胞因子之间的关系，以及中药对其的干预作用。
采用逆转录-聚合酶链反应（RT-PCR）法检测肺癌患者PBMC中Th1/Th2型细胞因子IFN-γ、IL-2、IL-4、IL-6、IL-10、转录因子T-bet/GATA3及肿瘤组织特异性mRNA CEA、CK19的基因表达。同时，观察肺癌患者PBMC经中药黄芪和川芎嗪培养前后IFN-γ、IL-4、T-bet和GATA3的变化。
42例肺癌患者中检测到Th2型细胞因子呈优势表达。IL-4、IL-6、IL-10、IFN-γ和IL-2的阳性率分别为27/42、24/42、31/42、4/42和5/42。而转录因子T-bet和GATA3的阳性率分别为16/42和34/42。此外，CEA和CK19阳性患者中T-bet的表达强度低于阴性患者。相反，GATA3在相同患者中的表达强度显著更高。
Th2型细胞因子的优势表达可能与T-bet表达降低或GATA3表达升高有关。这种情况可受到中药黄芪和川芎嗪的干预。
