Liu P, Collie N D
Department of Anatomy and Structural Biology, School of Medical Sciences, University of Otago, Dunedin, New Zealand.
Neuroscience. 2009 Sep 29;163(1):82-96. doi: 10.1016/j.neuroscience.2009.05.061. Epub 2009 May 29.
The present study systematically investigated the effects of agmatine administered i.p. in several commonly used behavioral tasks. In Experiment 1, pre-test treatment of agmatine (1 and 40 mg/kg) appeared to improve animals' performance in the water maze probe test conducted 24 h, but not 120 s, after training, when the effect was evaluated within subjects. In Experiment 2, pre-test agmatine treatment (40 mg/kg) did not affect animals' performance in the open field, and the place navigation, probe tests (1-4 and 6), reversal test and cued navigation in the water maze, but significantly facilitated performance in probe 5 which was conducted 96 h after training. In Experiment 3, rats with pre-test agmatine treatment (40 mg/kg) were less anxious relative to the controls, with no performance changes in the open field. In the water maze task, post-training agmatine treatment (40 mg/kg) did not affect place and cued navigation, but significantly improved animals' performance in the probe test conducted 24 h after training and the reversal test. In the working memory version of the task, agmatine treated rats took significantly less time and generated markedly shorter path length to reach the platform at the 180 s, but not 30 s, delay relative to the controls. In the object recognition task, rats with pre-test agmatine treatment (40 mg/kg) spent significantly more time exploring displaced objects, but not novel object, as compared to the controls. In Experiment 4, pre-test agmatine treatment (40 mg/kg) had no effect on the task acquisition in the delayed non-match to position task in the T-maze, but significantly facilitated performance at the 600 s delay. These results suggest that the behavioral effects of agmatine are task- and delay-dependent, and agmatine facilitates memory particularly when the task difficulty is increased due to memory trace decay and/or greater interference.
本研究系统地调查了腹腔注射胍丁胺在几种常用行为任务中的作用。在实验1中，当在个体内评估效果时，训练后24小时而非120秒进行的水迷宫探测测试中，预先给予胍丁胺（1和40毫克/千克）似乎能改善动物的表现。在实验2中，预先给予胍丁胺（40毫克/千克）对旷场试验、水迷宫中的位置导航、探测测试（1 - 4和6）、反转测试和线索导航中动物的表现没有影响，但显著促进了训练后96小时进行的探测5中的表现。在实验3中，预先给予胍丁胺（40毫克/千克）处理的大鼠相对于对照组焦虑程度更低，在旷场试验中表现没有变化。在水迷宫任务中，训练后给予胍丁胺（40毫克/千克）对位置和线索导航没有影响，但显著改善了训练后24小时进行的探测测试和反转测试中动物的表现。在该任务的工作记忆版本中，与对照组相比，胍丁胺处理的大鼠在180秒延迟而非30秒延迟时到达平台花费的时间显著更少，路径长度显著更短。在物体识别任务中，与对照组相比，预先给予胍丁胺（40毫克/千克）处理的大鼠花费显著更多时间探索被移动的物体，而非新物体。在实验4中，预先给予胍丁胺（40毫克/千克）对T迷宫中延迟位置匹配任务的任务获取没有影响，但在600秒延迟时显著促进了表现。这些结果表明，胍丁胺的行为效应取决于任务和延迟，并且胍丁胺尤其在由于记忆痕迹衰退和/或更大干扰导致任务难度增加时促进记忆。
