De-Deus Gustavo, de Souza Maria Claudia Brandão, Sergio Fidel Rivail Antonio, Fidel Sandra Rivera, de Campos Reinaldo Calixto, Luna Aderval S
Department of Endodontics, Rio de Janeiro State University, Rio de Janeiro, Brazil.
J Endod. 2009 Jun;35(6):887-90. doi: 10.1016/j.joen.2009.03.003.
This study was designed aiming to determine and compare the amount of arsenic in some brands of mineral trioxide aggregate (MTA) and Portland cement.
In the present study, arsenic species (As[III], As[V], and dimethylarsinic acid) were separated by high-performance liquid chromatography (HPLC) using a strong anion exchange column and converted into arsines by online HG. The instrumental coupling, HPLC-HG-AFS, was applied to 0.2 g of each cement that was prior digested in a solution of HCl, HNO(3), and HBF(4). Data were expressed as a part per million, and the preliminary analysis of the raw pooled data revealed a bell-shaped distribution. Statistical analysis was performed using one-way analysis of variance for multiple comparisons.
In all chromatograms obtained, only type III arsenic could be detected. The minimum amount of arsenic was detected in samples of white MTA ProRoot (3.3 x 10-4) and the maximum in the samples MTA Bio Angelus (Angelus, Londrina, PR, Brazil) (8.6 x 10-4). In the Gray MTA (Angelus), gray ProRoot MTA (Tulsa/Dentsply, Tulsa, OK) and CP Juntalider (Brasilatex Ltda, Diadema, SP, Brazil) did not detect any trace of arsenic. The values of arsenic found in CP Irajazinho (Votorantim Cimentos, Rio Branco, SP, Brazil) and white MTA Angelus were intermediaries to minimum and maximum values. The nonparametric test Kruskal-Wallis showed statistically similar results among all cements tested (p > 0.5).
Overall, the present study showed that all cements showed insignificant amounts of type III arsenic as well as no trace of arsenic DMA and type V could be detected.
本研究旨在测定并比较某些品牌的三氧化矿物凝聚体（MTA）和波特兰水泥中的砷含量。
在本研究中，使用强阴离子交换柱通过高效液相色谱法（HPLC）分离砷物种（As[III]、As[V]和二甲基砷酸），并通过在线氢化物发生（HG）将其转化为砷烷。将仪器联用HPLC-HG-AFS应用于0.2 g预先在HCl、HNO₃和HBF₄溶液中消化的每种水泥。数据以百万分之一表示，对原始汇总数据的初步分析显示呈钟形分布。使用单因素方差分析进行多重比较的统计分析。
在所有获得的色谱图中，仅能检测到III型砷。白色MTA ProRoot样品中检测到的砷含量最低（3.3×10⁻⁴），而MTA Bio Angelus（巴西朗德里纳的Angelus公司）样品中最高（8.6×10⁻⁴）。在灰色MTA（Angelus）、灰色ProRoot MTA（美国俄克拉何马州塔尔萨的登士柏国际公司）和CP Juntalider（巴西圣保罗迪亚德马的Brasilatex Ltda公司）中未检测到任何砷痕迹。在CP Irajazinho（巴西圣保罗里奥布兰科的Votorantim Cimentos公司）和白色MTA Angelus中发现的砷值介于最小值和最大值之间。非参数检验Kruskal-Wallis显示所有测试水泥之间的结果在统计学上相似（p>0.5）。
总体而言，本研究表明所有水泥中III型砷的含量均微不足道，并且未检测到砷DMA和V型砷的痕迹。
