Gajski Goran, Garaj-Vrhovac Vera
Institute for Medical Research and Occupational Health, Mutagenesis Unit, Ksaverska cesta 2, 10000 Zagreb, Croatia.
Int J Toxicol. 2009 Mar-Apr;28(2):88-98. doi: 10.1177/1091581809335051.
The aim of this study is to investigate the radioprotective effect of bee venom against DNA damage induced by 915-MHz microwave radiation (specific absorption rate of 0.6 W/kg) in Wistar rats. Whole blood lymphocytes of Wistar rats are treated with 1 microg/mL bee venom 4 hours prior to and immediately before irradiation. Standard and formamidopyrimidine-DNA glycosylase (Fpg)-modified comet assays are used to assess basal and oxidative DNA damage produced by reactive oxygen species. Bee venom shows a decrease in DNA damage compared with irradiated samples. Parameters of Fpg-modified comet assay are statistically different from controls, making this assay more sensitive and suggesting that oxidative stress is a possible mechanism of DNA damage induction. Bee venom is demonstrated to have a radioprotective effect against basal and oxidative DNA damage. Furthermore, bee venom is not genotoxic and does not produce oxidative damage in the low concentrations used in this study.
本研究旨在探讨蜂毒对915兆赫微波辐射（比吸收率为0.6瓦/千克）诱导的Wistar大鼠DNA损伤的辐射防护作用。Wistar大鼠的全血淋巴细胞在照射前4小时和照射前即刻用1微克/毫升蜂毒处理。采用标准和甲酰胺嘧啶 - DNA糖基化酶（Fpg）修饰的彗星试验评估活性氧产生的基础DNA损伤和氧化性DNA损伤。与照射样本相比，蜂毒使DNA损伤减少。Fpg修饰的彗星试验参数与对照组有统计学差异，使该试验更敏感，并表明氧化应激是DNA损伤诱导的一种可能机制。已证明蜂毒对基础DNA损伤和氧化性DNA损伤具有辐射防护作用。此外，在本研究使用的低浓度下，蜂毒没有遗传毒性且不会产生氧化损伤。
