Kalpana K B, Devipriya N, Srinivasan M, Menon Venugopal P
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar-608002, Tamil Nadu, India.
Mutat Res. 2009 May 31;676(1-2):54-61. doi: 10.1016/j.mrgentox.2009.03.005. Epub 2009 Mar 31.
The present study was aimed to evaluate the radioprotective efficacy of hesperidin (HN), a flavonone glycoside against gamma-radiation-induced cellular damage in cultured human peripheral blood lymphocytes. Different concentrations of HN (3.27, 6.55, 9.83, 13.10, 16.38 and 19.65 microM) were pre-incubated with lymphocytes for 30 min prior to gamma-irradiation [4 Gy] and the micronuclei (MN) scoring, dicentric aberration and comet assay were performed to fix the effective dose of HN against gamma-irradiation induced cellular damage. The results indicated that among all the concentrations, 16.38 microM concentration of HN showed optimum protection by effectively decreasing the MN frequencies, dicentric aberrations and comet attributes. Based on the above results, 16.38 microM concentration of HN was fixed as the effective dose to further investigate its radioprotective efficacy which was then carried out by pre-incubating lymphocytes with 16.38 microM concentration of HN, exposing the lymphocytes to different doses (1, 2, 3 and 4 Gy) of radiation and investigating radiation induced genetic damage (MN, dicentric aberration, comet assay, DNA fragmentation assay) and biochemical changes (changes in the level of enzymic and non-enzymic antioxidants, lipid peroxidation). The results indicated a dose dependent increase in both genetic damage and thiobarbituric acid reactive substances (TBARS), accompanied by a significant decrease in the antioxidant status compared to HN treated groups which modulated the toxic effects through its antioxidant potential. Thus the current study shows HN to be an effective radioprotector against gamma-radiation induced in-vitro cellular damage in lymphocytes.
本研究旨在评估橙皮苷（HN），一种黄酮苷对培养的人外周血淋巴细胞中γ射线诱导的细胞损伤的辐射防护效果。在γ射线照射[4 Gy]前，将不同浓度的HN（3.27、6.55、9.83、13.10、16.38和19.65微摩尔）与淋巴细胞预孵育30分钟，然后进行微核（MN）评分、双着丝粒畸变分析和彗星试验，以确定HN对γ射线诱导的细胞损伤的有效剂量。结果表明，在所有浓度中，16.38微摩尔浓度的HN通过有效降低MN频率、双着丝粒畸变和彗星试验指标显示出最佳保护作用。基于上述结果，将16.38微摩尔浓度的HN确定为有效剂量，以进一步研究其辐射防护效果，随后通过将淋巴细胞与16.38微摩尔浓度的HN预孵育，使淋巴细胞暴露于不同剂量（1、2、3和4 Gy）的辐射下，并研究辐射诱导的遗传损伤（MN、双着丝粒畸变、彗星试验、DNA片段化试验）和生化变化（酶促和非酶促抗氧化剂水平的变化、脂质过氧化）。结果表明，与通过其抗氧化潜力调节毒性作用的HN处理组相比，遗传损伤和硫代巴比妥酸反应性物质（TBARS）均呈剂量依赖性增加，同时抗氧化状态显著降低。因此，本研究表明HN是一种有效的辐射防护剂，可对抗γ射线诱导的淋巴细胞体外细胞损伤。
