Matos L, Stevenson D, Gomes F, Silva-Carvalho J L, Almeida H
Laboratory of Cell and Molecular Biology, Faculty of Medicine, University of Porto, Porto, Portugal.
Mol Hum Reprod. 2009 Jul;15(7):411-9. doi: 10.1093/molehr/gap034. Epub 2009 May 29.
Success in assisted reproductive techniques (ART) is influenced by gamete and embryo quality but the assessment of these parameters has been thwarted by the lack of reliable biomarkers. Follicular fluid and cumulus oophorus cells may provide biomarkers due to their close relationship to the oocyte. These cells produce antioxidants and thus protect the oocyte from oxidative damage exerted by reactive oxygen species (ROS). ROS and antioxidants are known to intervene in reproductive physiology and pathology, but their roles are unclear. It is hypothesized that superoxide dismutase (SOD), a first line antioxidant enzyme, is associated with oocyte quality. Cells obtained in the course of ART for the treatment of infertility due to male factor or female pathology were processed for SOD intracellular isoforms (CuZnSOD and MnSOD) immunodetection, total SOD activity and isoforms content. Cells presented strong positive staining for CuZnSOD and MnSOD. SOD activity decreased with increasing female age but was increased in endometriosis and in ovulatory dysfunction. When male factor was the cause for infertility, successful ART was associated with higher SOD activity. Variations in SOD emphasize the relevance of oxidative stress in the oocyte maturation process. These variations also suggest that SOD is a potential biomarker for ART success.
辅助生殖技术（ART）的成功受到配子和胚胎质量的影响，但由于缺乏可靠的生物标志物，这些参数的评估一直受到阻碍。卵泡液和卵丘细胞可能因其与卵母细胞的密切关系而提供生物标志物。这些细胞产生抗氧化剂，从而保护卵母细胞免受活性氧（ROS）造成的氧化损伤。已知ROS和抗氧化剂会干预生殖生理和病理过程，但其作用尚不清楚。据推测，超氧化物歧化酶（SOD）作为一线抗氧化酶，与卵母细胞质量相关。对因男性因素或女性病理因素导致不孕而接受ART治疗过程中获得的细胞进行处理，以检测SOD细胞内同工型（CuZnSOD和MnSOD）的免疫反应、总SOD活性和同工型含量。细胞对CuZnSOD和MnSOD呈现强阳性染色。SOD活性随女性年龄增长而降低，但在子宫内膜异位症和排卵功能障碍中升高。当男性因素导致不孕时，ART成功与较高的SOD活性相关。SOD的变化强调了氧化应激在卵母细胞成熟过程中的相关性。这些变化还表明SOD是ART成功的潜在生物标志物。
