Takahashi Norihisa, Chikama Tai-Ichiro, Yanai Ryoji, Nishida Teruo
Department of Ophthalmology, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan.
Jpn J Ophthalmol. 2009 May;53(3):199-203. doi: 10.1007/s10384-008-0661-4. Epub 2009 May 31.
To examine the relations between specific structures detected at the corneal limbus by confocal biomicroscopy with palisades of Vogt detected by slit-lamp microscopy
Twenty-five eyes of 25 normal participants were examined. The presence or absence of the palisades of Vogt and limbal pigmentation were assessed by slit-lamp microscopy. The presence or absence of four structures in the corneal limbal area--hyperreflective parallel trabecular extensions, borders of bright-cell groups and dark-cell groups, hyperreflective cells and bright corpuscular particles with dendritic cell-like morphology--were assessed by confocal biomicroscopy. The relations between the presence of these four structures and the presence of either the palisades of Vogt or limbal pigmentation of the palisade ridges were evaluated by the chi-squared test.
Only the presence of hyperreflective parallel trabecular extensions was significantly related to the presence of the palisades of Vogt (P < 0.01). The presence of hyperreflective cells was not significantly related to the presence of limbal pigmentation.
The hyperreflective parallel trabecular extensions observed by confocal biomicroscopy may correspond to the palisades of Vogt detected by slit-lamp microscopy. Further studies are needed to determine whether confocal biomicroscopy can be used to diagnose and monitor the clinical course of limbal stem cell deficiency.
通过共焦显微镜检查在角膜缘检测到的特定结构与通过裂隙灯显微镜检查检测到的沃格特栅栏之间的关系。
对25名正常受试者的25只眼睛进行检查。通过裂隙灯显微镜评估沃格特栅栏的存在与否以及角膜缘色素沉着情况。通过共焦显微镜评估角膜缘区域中四种结构的存在与否，即高反射平行小梁延伸、亮细胞群和暗细胞群的边界、高反射细胞以及具有树突状细胞样形态的亮小球颗粒。通过卡方检验评估这四种结构的存在与沃格特栅栏或栅栏嵴的角膜缘色素沉着的存在之间的关系。
仅高反射平行小梁延伸的存在与沃格特栅栏的存在显著相关（P < 0.01）。高反射细胞的存在与角膜缘色素沉着的存在无显著相关性。
共焦显微镜观察到的高反射平行小梁延伸可能对应于裂隙灯显微镜检测到的沃格特栅栏。需要进一步研究以确定共焦显微镜是否可用于诊断和监测角膜缘干细胞缺乏的临床病程。
