Nitschke M, Araújo L V, Costa S G V A O, Pires R C, Zeraik A E, Fernandes A C L B, Freire D M G, Contiero J
University of São Paulo, Chemistry Institute of São Carlos, Avenida Trabalhador São-Carlense, São Carlos, SP, Brazil.
Lett Appl Microbiol. 2009 Aug;49(2):241-7. doi: 10.1111/j.1472-765X.2009.02646.x. Epub 2009 May 22.
To investigate the effect of the biosurfactants surfactin and rhamnolipids on the adhesion of the food pathogens Listeria monocytogenes, Enterobacter sakazakii and Salmonella Enteritidis to stainless steel and polypropylene surfaces.
Quantification of bacterial adhesion was performed using the crystal violet staining technique. Preconditioning of surfaces with surfactin caused a reduction on the number of adhered cells of Ent. sakazakii and L. monocytogenes on stainless steel. The most significant result was obtained with L. monocytogenes where number of adhered cells was reduced by 10(2) CFU cm(-2). On polypropylene, surfactin showed a significant decrease on the adhesion of all strains. The adsorption of surfactin on polystyrene also reduces the adhesion of L. monocytogenes and Salm. Enteritidis growing cells. For short contact periods using nongrowing cells or longer contact periods with growing cells, surfactin was able to delay bacterial adhesion.
The prior adsorption of surfactin to solid surfaces contributes on reducing colonization of the pathogenic bacteria.
This is the first work investigating the effect of surfactin on the adhesion of the food pathogens L. monocytogenes, Ent. sakazakii and Salm. Enteritidis to polypropylene and stainless steel surfaces.
研究生物表面活性剂表面活性素和鼠李糖脂对食源性病原体单核细胞增生李斯特菌、阪崎肠杆菌和肠炎沙门氏菌在不锈钢和聚丙烯表面黏附的影响。
采用结晶紫染色技术对细菌黏附进行定量分析。用表面活性素预处理表面可使阪崎肠杆菌和单核细胞增生李斯特菌在不锈钢表面的黏附细胞数量减少。对单核细胞增生李斯特菌的效果最为显著，其黏附细胞数量减少了10(2) CFU cm(-2)。在聚丙烯表面，表面活性素使所有菌株的黏附力均显著下降。表面活性素在聚苯乙烯上的吸附也降低了单核细胞增生李斯特菌和肠炎沙门氏菌生长细胞的黏附。对于使用非生长细胞的短接触时间或使用生长细胞的长接触时间，表面活性素能够延迟细菌黏附。
表面活性素预先吸附到固体表面有助于减少病原菌的定殖。
这是第一项研究表面活性素对食源性病原体单核细胞增生李斯特菌、阪崎肠杆菌和肠炎沙门氏菌在聚丙烯和不锈钢表面黏附影响的工作。
