动物源凝固酶阴性葡萄球菌中葡萄球菌盒式染色体的多样性。
Diversity of staphylococcal cassette chromosome in coagulase-negative staphylococci from animal sources.
机构信息
Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, OH, USA.
出版信息
J Appl Microbiol. 2009 Oct;107(4):1375-83. doi: 10.1111/j.1365-2672.2009.04322.x. Epub 2009 Apr 18.
AIMS
To type the staphylococcal cassette chromosome (SCC) in coagulase-negative staphylococci (CoNS) from animal sources.
METHODS AND RESULTS
A total of 92 CoNS isolates recovered from farm animals was analysed. The top three staphylococcal species were Staphylococcus lentus (34), S. sciuri (31), and S. xylosus (13). The presence of the cassette chromosome recombinase (ccr) genes ccrA1, ccrB1, ccrA2, ccrB2, ccrA3, ccrB3 and ccrC, the mec regulatory genes mecI and mecR1, and Tn554 was used to differentiate the SCC. A total of 60 of the 92 isolates were methicillin resistant. Among the 60 methicillin-resistant Staphylococcus spp. isolates, SCCmec (mecA-carrying SCC) types I, III, IV and V were identified in 24 isolates based on the combinations of the ccr genes and the mec regulatory genes, with type III being predominant. The single S. epidermidis carried SCCmec type IV. SCC type III was also identified in two of 32 methicillin-susceptible isolates. Identical SCCmec types were present in different species of CoNS. Pulsed-field gel electrophoresis (PFGE) generated 64 patterns out of 81 PFGE typeable isolates. Indistinguishable clones were detected in animals from different farms.
CONCLUSIONS
Heterogeneous SCC existed in CoNS of diverse genetic background. Both clonal transmission of methicillin-resistant CoNS and horizontal transfer of SCCmec occurred in the animal production environment.
SIGNIFICANCE AND IMPACT OF THE STUDY
This study adds to our knowledge of SCCmec type and the diversity of SCC in CoNS.
目的
对动物源凝固酶阴性葡萄球菌（CoNS）中的葡萄球菌盒式染色体（SCC）进行分型。
方法和结果
共分析了 92 株从农场动物中分离得到的 CoNS 菌株。排名前三的葡萄球菌物种分别是迟缓葡萄球菌（34 株）、松鼠葡萄球菌（31 株）和木糖葡萄球菌（13 株）。采用盒式染色体重组酶（ccr）基因 ccrA1、ccrB1、ccrA2、ccrB2、ccrA3、ccrB3 和 ccrC、mec 调节基因 mecI 和 mecR1 以及 Tn554 的存在来区分 SCC。92 株分离株中共有 60 株为耐甲氧西林。在这 60 株耐甲氧西林金黄色葡萄球菌中，根据 ccr 基因和 mec 调节基因的组合，在 24 株分离株中鉴定出 SCCmec（携带 mecA 的 SCC）I、III、IV 和 V 型，其中以 III 型为主。唯一一株表皮葡萄球菌携带 SCCmec 型 IV。在 32 株甲氧西林敏感的分离株中也鉴定出了两种 SCC 型 III。不同 CoNS 物种中存在相同的 SCCmec 型。81 株可进行脉冲场凝胶电泳（PFGE）的分离株产生了 64 种 PFGE 型。在来自不同农场的动物中检测到了相同的克隆。
结论
不同遗传背景的 CoNS 中存在异质性 SCC。耐甲氧西林 CoNS 的克隆传播和 SCCmec 的水平转移都发生在动物生产环境中。
研究的意义和影响
本研究增加了我们对 CoNS 中 SCCmec 型和 SCC 多样性的了解。
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