Technical Institution of Dentistry, University of Hama, Hama, Syria.
Sir John Walsh Research Institute, University of Otago, Dunedin, New Zealand.
BMC Oral Health. 2019 May 2;19(1):71. doi: 10.1186/s12903-019-0765-x.
This study evaluated the efficacy of four methods in removing Candida albicans from the acrylic base material used to fabricate removable orthodontic appliances.
Heat-processed bars of orthodontic acrylic were incubated in a suspension of C. albicans for 2 h at 37 °C. Samples were allocated into five groups (five bars per group) according to the cleaning method: (1) manual brushing using a toothbrush; (2) soaking in a commercial denture cleaning solution; (3) soaking in a commercial mouthwash solution; (4) using an ultrasonic cleaner; and (5) soaking in distilled water as a negative control. Yeast remaining attached to the bars after cleaning were removed by vortexing in growth medium and plated on Sabouraud dextrose agar. The reduction in yeast colony count after cleaning was calculated and expressed as the number of colony forming units per acrylic bar (CFU/bar). The experiment was carried out three times.
All four cleaning methods resulted in a significant decrease in viable yeast cells associated with the acrylic bars compared to the control group. The mean percentage reduction in viable yeast cells affected by the cleaning methods was: brushing 89.9%; chlorhexidine 95.8%; ultrasonic cleaning 99.9%; and denture tablet 100%.
All four methods evaluated in this study were effective, to some extent, in removing C. albicans from orthodontic acrylic samples. The most effective, and readily available, cleaning method was the use of commercial denture sterilizing tablets.
本研究评估了四种方法从用于制作可摘正畸矫治器的丙烯酸基材料中去除白色念珠菌的功效。
将正畸丙烯酸热加工棒在 37°C 的白色念珠菌悬浮液中孵育 2 小时。根据清洁方法将样品分配到五个组（每组五个棒）：（1）用牙刷手动刷洗；（2）浸泡在商业义齿清洁剂中；（3）浸泡在商业漱口水溶液中；（4）使用超声波清洗器；（5）浸泡在蒸馏水中作为阴性对照。清洁后，用生长培养基涡旋去除附着在棒上的剩余酵母，并在萨布罗琼脂上平板。计算清洁后酵母菌落计数的减少，并表示为每根丙烯酸棒的菌落形成单位数（CFU/棒）。该实验进行了三次。
与对照组相比，所有四种清洁方法均导致与丙烯酸棒相关的存活酵母细胞数量显著减少。清洁方法对存活酵母细胞的平均减少百分比为：刷洗 89.9%；洗必泰 95.8%；超声清洗 99.9%；和假牙片 100%。
本研究评估的所有四种方法在某种程度上都能有效去除正畸丙烯酸样本中的白色念珠菌。最有效且易于获得的清洁方法是使用商业假牙消毒片。
