Song Lan, Xu Zhaojun, Zhang Caiping, Qiao Xinhui, Huang Chunling
Laboratory of Shock, Department of Pathophysiology, Xiangya School of Medicine, Central South University, 110 Xiangya Road, Changsha, Hunan 410078, People's Republic of China.
Biochem Biophys Res Commun. 2009 Aug 14;386(1):30-4. doi: 10.1016/j.bbrc.2009.05.120. Epub 2009 May 30.
Forkhead box protein A1 (Foxa1) is an evolutionarily conserved winged helix transcription factor. In this study, the effect of Foxa1 on the expression of HSP72 was examined by RT-PCR and Western blot in Foxa1 overexpression or deficient cells. The results showed overexpression of Foxa1 promoted the expression of HSP72, while Foxa1 depletion, induced by antisense oligonucleotides, decreased the expression of HSP72 in MCF-7 cells under normal and heat stress condition. Electrophoretic mobility shift assay and chromatin immunoprecipitation revealed that Foxa1 bound to HSP72 promoter, and heat stress promoted its DNA binding activity. Luciferase reporter showed that Foxa1 also increased the transcription activity of HSP72 promoter. These results indicate an important role for Foxa1 as a novel regulator of expression of HSP72.
叉头框蛋白A1（Foxa1）是一种进化上保守的翼状螺旋转录因子。在本研究中，通过RT-PCR和蛋白质免疫印迹法检测了Foxa1在过表达或缺失的细胞中对HSP72表达的影响。结果显示，Foxa1的过表达促进了HSP72的表达，而反义寡核苷酸诱导的Foxa1缺失则降低了MCF-7细胞在正常和热应激条件下HSP72的表达。电泳迁移率变动分析和染色质免疫沉淀显示，Foxa1与HSP72启动子结合，热应激促进其DNA结合活性。荧光素酶报告基因显示，Foxa1还增加了HSP72启动子的转录活性。这些结果表明，Foxa1作为HSP72表达的新型调节因子具有重要作用。
