Department of Biochemistry and Molecular Biology, Hunan University of Chinese Medicine, Changsha, Hunan, 410208, China,
Cell Stress Chaperones. 2014 Jan;19(1):53-60. doi: 10.1007/s12192-013-0433-z. Epub 2013 Apr 28.
Forkhead box protein A1 (FoxA1) is a transcription factor that is involved in embryonic development and cell differentiation. In this study, we show that hydrogen peroxide (H2O2) treatment upregulated expression of FoxA1 and UCP2 in the A549 cell line. Overexpression of FoxA1 by full-length complementary DNA reduced UCP2 expression, while silencing of FoxA1 expression by small interfering RNA significantly increased UCP2 levels. FoxA1 binds to a site from -919 to -913 bp relative to the UCP2 transcription start site. The overexpression of FoxA1 promoted the DNA binding activity and attenuated the transcription of UCP2 promoter as shown by electromobility shift, chromatin immunoprecipitation assays, and luciferase reporter assay. These data indicate an important role of FoxA1 in regulating expression of UCP2.
叉头框蛋白 A1（FoxA1）是一种转录因子，参与胚胎发育和细胞分化。在这项研究中，我们表明过氧化氢（H2O2）处理上调了 A549 细胞系中 FoxA1 和 UCP2 的表达。全长 cDNA 过表达 FoxA1 降低了 UCP2 的表达，而 FoxA1 表达的小干扰 RNA 沉默则显著增加了 UCP2 的水平。FoxA1 与 UCP2 转录起始位点相对的-919 至-913bp 处的一个位点结合。FoxA1 的过表达促进了 UCP2 启动子的 DNA 结合活性，并减弱了其转录，如电泳迁移率变动、染色质免疫沉淀测定和荧光素酶报告基因测定所示。这些数据表明 FoxA1 在调节 UCP2 的表达中起着重要作用。
