叉头框蛋白 A1 抑制过氧化氢诱导的 A549 细胞系中解偶联蛋白 2 的表达。
Forkhead box protein A1 inhibits the expression of uncoupling protein 2 in hydrogen peroxide-induced A549 cell line.
机构信息
Department of Biochemistry and Molecular Biology, Hunan University of Chinese Medicine, Changsha, Hunan, 410208, China,
出版信息
Cell Stress Chaperones. 2014 Jan;19(1):53-60. doi: 10.1007/s12192-013-0433-z. Epub 2013 Apr 28.
Abstract
Forkhead box protein A1 (FoxA1) is a transcription factor that is involved in embryonic development and cell differentiation. In this study, we show that hydrogen peroxide (H2O2) treatment upregulated expression of FoxA1 and UCP2 in the A549 cell line. Overexpression of FoxA1 by full-length complementary DNA reduced UCP2 expression, while silencing of FoxA1 expression by small interfering RNA significantly increased UCP2 levels. FoxA1 binds to a site from -919 to -913 bp relative to the UCP2 transcription start site. The overexpression of FoxA1 promoted the DNA binding activity and attenuated the transcription of UCP2 promoter as shown by electromobility shift, chromatin immunoprecipitation assays, and luciferase reporter assay. These data indicate an important role of FoxA1 in regulating expression of UCP2.
摘要
叉头框蛋白 A1（FoxA1）是一种转录因子，参与胚胎发育和细胞分化。在这项研究中，我们表明过氧化氢（H2O2）处理上调了 A549 细胞系中 FoxA1 和 UCP2 的表达。全长 cDNA 过表达 FoxA1 降低了 UCP2 的表达，而 FoxA1 表达的小干扰 RNA 沉默则显著增加了 UCP2 的水平。FoxA1 与 UCP2 转录起始位点相对的-919 至-913bp 处的一个位点结合。FoxA1 的过表达促进了 UCP2 启动子的 DNA 结合活性，并减弱了其转录，如电泳迁移率变动、染色质免疫沉淀测定和荧光素酶报告基因测定所示。这些数据表明 FoxA1 在调节 UCP2 的表达中起着重要作用。
相似文献
1
Forkhead box protein A1 inhibits the expression of uncoupling protein 2 in hydrogen peroxide-induced A549 cell line.叉头框蛋白 A1 抑制过氧化氢诱导的 A549 细胞系中解偶联蛋白 2 的表达。
Cell Stress Chaperones. 2014 Jan;19(1):53-60. doi: 10.1007/s12192-013-0433-z. Epub 2013 Apr 28.
2
Transcriptional co-activator peroxisome proliferator-activated receptor (PPAR)gamma co-activator-1beta is involved in the regulation of glucose-stimulated insulin secretion in INS-1E cells.转录共激活因子过氧化物酶体增殖物激活受体（PPAR）γ共激活因子-1β参与INS-1E细胞中葡萄糖刺激的胰岛素分泌调节。
J Mol Med (Berl). 2009 Mar;87(3):299-306. doi: 10.1007/s00109-008-0425-0. Epub 2008 Dec 11.
3
Up-regulation of the HSP72 by Foxa1 in MCF-7 human breast cancer cell line.在MCF-7人乳腺癌细胞系中，Foxa1对热休克蛋白72（HSP72）的上调作用。
Biochem Biophys Res Commun. 2009 Aug 14;386(1):30-4. doi: 10.1016/j.bbrc.2009.05.120. Epub 2009 May 30.
4
Role of Foxa1 in regulation of bcl2 expression during oxidative-stress-induced apoptosis in A549 type II pneumocytes.叉头框蛋白A1（Foxa1）在氧化应激诱导的A549 II型肺细胞凋亡过程中对bcl2表达的调控作用。
Cell Stress Chaperones. 2009 Jul;14(4):417-25. doi: 10.1007/s12192-008-0095-4. Epub 2009 Jan 7.
5
Increased hepatic UCP2 expression in rats with nonalcoholic steatohepatitis is associated with upregulation of Sp1 binding to its motif within the proximal promoter region.非酒精性脂肪性肝炎大鼠肝脏中解偶联蛋白2（UCP2）表达增加，与近端启动子区域内Sp1与其基序结合的上调有关。
J Cell Biochem. 2008 Sep 1;105(1):277-89. doi: 10.1002/jcb.21827.
6
Forkhead box A1 (FOXA1) and A2 (FOXA2) oppositely regulate human type 1 iodothyronine deiodinase gene in liver.叉头框转录因子 A1（FOXA1）和 A2（FOXA2）在肝脏中对人甲状腺素 1 型脱碘酶基因起相反调节作用。
Endocrinology. 2012 Jan;153(1):492-500. doi: 10.1210/en.2011-1310. Epub 2011 Nov 8.
7
MicroRNA-93 Promotes Epithelial-Mesenchymal Transition of Endometrial Carcinoma Cells.微小RNA-93促进子宫内膜癌细胞的上皮-间质转化
PLoS One. 2016 Nov 9;11(11):e0165776. doi: 10.1371/journal.pone.0165776. eCollection 2016.
8
Role of mitochondrial uncoupling protein 2 in cancer cell resistance to gemcitabine.线粒体解偶联蛋白2在癌细胞对吉西他滨耐药中的作用
Biochim Biophys Acta. 2012 Oct;1823(10):1856-63. doi: 10.1016/j.bbamcr.2012.06.007. Epub 2012 Jun 15.
9
Negative regulation of UCP2 by TGFβ signaling characterizes low and intermediate-grade primary breast cancer.TGFβ 信号通路对 UCP2 的负调控作用是低级别和中级别原发性乳腺癌的特征。
Cell Death Dis. 2010 Jul 15;1(7):e53. doi: 10.1038/cddis.2010.30.
10
BRCA1 and FOXA1 proteins coregulate the expression of the cell cycle-dependent kinase inhibitor p27(Kip1).BRCA1蛋白和FOXA1蛋白共同调节细胞周期依赖性激酶抑制剂p27（Kip1）的表达。
Oncogene. 2006 Mar 2;25(9):1391-9. doi: 10.1038/sj.onc.1209170.
引用本文的文献
1
Mitochondrial uncoupling protein 2: a central player in pancreatic disease pathophysiology.线粒体解偶联蛋白2：胰腺疾病病理生理学的核心参与者。
Mol Med. 2024 Dec 20;30(1):259. doi: 10.1186/s10020-024-01027-y.
2
Emerging role and the signaling pathways of uncoupling protein 2 in kidney diseases.解偶联蛋白 2 在肾脏疾病中的作用及信号通路。
Ren Fail. 2024 Dec;46(2):2381604. doi: 10.1080/0886022X.2024.2381604. Epub 2024 Aug 1.
3
Systematic Pan-Cancer Analysis and Experimental Verification Identify FOXA1 as an Immunological and Prognostic Biomarker in Epithelial Ovarian Cancer.系统泛癌症分析和实验验证将 FOXA1 鉴定为上皮性卵巢癌中的免疫和预后生物标志物。
Dis Markers. 2022 Nov 2;2022:9328972. doi: 10.1155/2022/9328972. eCollection 2022.
4
Factors affecting expression and transcription of uncoupling protein 2 gene.影响解偶联蛋白 2 基因表达和转录的因素。
J Vet Med Sci. 2020 Dec 26;82(12):1734-1741. doi: 10.1292/jvms.20-0444. Epub 2020 Nov 7.
5
The potential mechanism of mitochondrial dysfunction in septic cardiomyopathy.脓毒症性心肌病中线粒体功能障碍的潜在机制。
J Int Med Res. 2018 Jun;46(6):2157-2169. doi: 10.1177/0300060518765896. Epub 2018 Apr 11.
本文引用的文献
1
Critical role of H2O2 generated by NOX4 during cellular response under glucose deprivation.在葡萄糖剥夺下细胞反应中由 NOX4 产生的 H2O2 的关键作用。
PLoS One. 2013;8(3):e56628. doi: 10.1371/journal.pone.0056628. Epub 2013 Mar 21.
2
Loss of the urothelial differentiation marker FOXA1 is associated with high grade, late stage bladder cancer and increased tumor proliferation.尿路上皮分化标志物 FOXA1 的丢失与高级别、晚期膀胱癌以及肿瘤增殖增加相关。
PLoS One. 2012;7(5):e36669. doi: 10.1371/journal.pone.0036669. Epub 2012 May 10.
3
FOXA1 represses the molecular phenotype of basal breast cancer cells.FOXA1 抑制基底型乳腺癌细胞的分子表型。
Oncogene. 2013 Jan 31;32(5):554-63. doi: 10.1038/onc.2012.62. Epub 2012 Mar 5.
4
Opposite roles of FOXA1 and NKX2-1 in lung cancer progression.FOXA1 和 NKX2-1 在肺癌进展中的相反作用。
Genes Chromosomes Cancer. 2012 Jun;51(6):618-29. doi: 10.1002/gcc.21950. Epub 2012 Mar 2.
5
Forkhead box transcription factor, forkhead box A1, shows negative association with lymph node status in endometrial cancer, and represses cell proliferation and migration of endometrial cancer cells.叉头框转录因子 A1 与子宫内膜癌的淋巴结状态呈负相关，并抑制子宫内膜癌细胞的增殖和迁移。
Cancer Sci. 2012 Apr;103(4):806-12. doi: 10.1111/j.1349-7006.2012.02201.x. Epub 2012 Feb 7.
6
UCP2 inhibits ROS-mediated apoptosis in A549 under hypoxic conditions.UCP2 抑制低氧条件下 A549 细胞中 ROS 介导的细胞凋亡。
PLoS One. 2012;7(1):e30714. doi: 10.1371/journal.pone.0030714. Epub 2012 Jan 24.
7
FOXA1 is an independent prognostic marker for ER-positive breast cancer.FOXA1 是 ER 阳性乳腺癌的独立预后标志物。
Breast Cancer Res Treat. 2012 Feb;131(3):881-90. doi: 10.1007/s10549-011-1482-6. Epub 2011 Apr 19.
8
Negative regulation of UCP2 by TGFβ signaling characterizes low and intermediate-grade primary breast cancer.TGFβ 信号通路对 UCP2 的负调控作用是低级别和中级别原发性乳腺癌的特征。
Cell Death Dis. 2010 Jul 15;1(7):e53. doi: 10.1038/cddis.2010.30.
9
Mitochondrial uncoupling protein 2 expression in colon cancer and its clinical significance.结肠癌中线粒体解偶联蛋白 2 的表达及其临床意义。
World J Gastroenterol. 2010 Dec 7;16(45):5773-8. doi: 10.3748/wjg.v16.i45.5773.
10
Mitochondrial uncoupling inhibits p53 mitochondrial translocation in TPA-challenged skin epidermal JB6 cells.线粒体解偶联抑制 TPA 刺激的皮肤表皮 JB6 细胞中 p53 的线粒体易位。
PLoS One. 2010 Oct 18;5(10):e13459. doi: 10.1371/journal.pone.0013459.
Zotero 插件