Stange E, Papenberg J, Agostini B
Histochemistry. 1977 May 20;52(2):145-9. doi: 10.1007/BF00492290.
Iodination of proteins and lipoproteins is a widely used "in vitro" labelling procedure in metabolic, autoradiographic and various other studies. However, all available iodination techniques have involved the possible damage to the proteins by self-irradiation, oxidizing agents, the alkaline milieu or by the introduction of iodine into the molecular structure itself. To evaluate the integrity of iodinated lipoprotiens, we observed the electron microscopic appearance of normal and iodinated rabbit very low density lipoproteins (VLDL) by negative staining with phosphotungstic acid. Iodination up to a molar iodine/protein ratio of 2.89 did not results in any change of shape, size or aggregating tendency of the particles. No stacks or disk-like particles like those of various hyperlipoproteinemic states were found. We conclude that electron microscopy is a valuable tool in assessing the morphological appearance of lipoprotein iodination, but it should be complemented by other techniques.
蛋白质和脂蛋白的碘化是代谢、放射自显影及其他各类研究中广泛应用的一种“体外”标记方法。然而，所有现有的碘化技术都可能因自身辐射、氧化剂、碱性环境或碘引入分子结构本身而对蛋白质造成损害。为评估碘化脂蛋白的完整性，我们通过磷钨酸负染观察了正常及碘化兔极低密度脂蛋白（VLDL）的电子显微镜外观。碘化至碘/蛋白质摩尔比达2.89时，颗粒的形状、大小或聚集倾向均未发生任何变化。未发现各种高脂蛋白血症状态下出现的堆叠或盘状颗粒。我们得出结论，电子显微镜是评估脂蛋白碘化形态外观的一种有价值的工具，但应辅以其他技术。
