Vermeer B J, De Bruijn W C, Van Gent C M, De Winter C P
Histochem J. 1978 May;10(3):299-307. doi: 10.1007/BF01007561.
The application of OSO4 plus K3 [Fe(CN)6] as a secondary fixative following aldehyde fixation, permitted demonstration of the presence of 30-300 nm 'membrane-bound' particles in xanthomatous tissue. With the same fixation method, isolated low density lipoprotein particles in a fibrin matrix could be observed in the transmission electron microscope in a way permitting comparison with similarly fixed tissue. However, isolated particles of very low density lipoproteins treated in the same way as low density particles had an irregular appearance and a diameter varying between 30 and 80 nm.
在醛类固定之后，使用四氧化锇（OSO4）加铁氰化钾（K3[Fe(CN)6]）作为二次固定剂，能够证实在黄色瘤组织中存在30 - 300纳米的“膜结合”颗粒。采用相同的固定方法，在透射电子显微镜下可以观察到纤维蛋白基质中分离的低密度脂蛋白颗粒，其观察方式便于与同样固定的组织进行比较。然而，以与低密度颗粒相同的方式处理的极低密度脂蛋白分离颗粒外观不规则，直径在30至80纳米之间变化。
