Department of Biology, Duke University, Durham, North Carolina 27708, USA.
Genetics. 2009 Aug;182(4):1377-9. doi: 10.1534/genetics.109.103069. Epub 2009 Jun 1.
We describe a simple genetic test for assessing the competency of Gal4-based baits prior to a yeast two-hybrid screen, which allows determination of whether a bait protein is expressed appropriately for an interaction to be detected. The novel test, based on interaction with the protein RanBPM, is easier and more predictive than other methods such as Western blotting, allowing identification of approximately 80% of incompetent baits prior to screening.
我们描述了一种简单的遗传测试方法，用于在酵母双杂交筛选之前评估 Gal4 诱饵的效力，该方法可确定诱饵蛋白是否表达适当，以检测相互作用。与 RanBPM 蛋白相互作用的新测试比 Western blot 等其他方法更简单、更具预测性，可在筛选前鉴定约 80%的无效诱饵。
