Dogan Mehmet, Aksun M Irsadi, Swan Anna K, Goldberg Bennett B, Unlü M Selim
Physics Department, Boston University, 590 Commonwealth Avenue, Boston, Massachusetts 02215, USA.
J Opt Soc Am A Opt Image Sci Vis. 2009 Jun;26(6):1458-66. doi: 10.1364/josaa.26.001458.
Dipole radiation in and near planar stratified dielectric media is studied theoretically within the context of fluorescence microscopy, as fluorescent emitters are generally modeled by electric dipoles. Although the main emphasis of this study is placed on the closed-form representations of the field components of fluorescent emitters in layered environments in near- and far-field regions, the underlying motive is to understand the limits of spectral self-interference fluorescence microscopy in studying the dipole orientation of fluorophores. Since accurate calculations of the field components of arbitrarily polarized electric dipoles in layered environments are computationally very time-consuming, a method for finding their closed-form representations is proposed using the closed-form potential Green's functions previously developed for microwave applications. The method is verified on typical geometries used in spectral self-interference microscopy experiments, where a dipole emitter is positioned over a slab of SiO(2) on top of a Si substrate. In addition to facilitating efficient calculation of near and intermediate fields of fluorescent emitters, closed-form Green's functions for fields would also play a crucial role in developing efficient and rigorous computational analysis and design tools for optical passive devices such as optical antennas by significantly improving the computational cost of the numerical solution of the integral equation.
在荧光显微镜的背景下,从理论上研究了平面分层介质内部及附近的偶极辐射,因为荧光发射体通常用电偶极来建模。尽管本研究的主要重点是近场和远场区域中分层环境下荧光发射体场分量的封闭形式表示,但潜在目的是了解光谱自干涉荧光显微镜在研究荧光团偶极取向方面的局限性。由于在分层环境中精确计算任意极化电偶极的场分量在计算上非常耗时,因此提出了一种利用先前为微波应用开发的封闭形式势格林函数来找到其封闭形式表示的方法。该方法在光谱自干涉显微镜实验中使用的典型几何结构上得到了验证,其中一个偶极发射体位于硅衬底上的二氧化硅平板上方。除了便于高效计算荧光发射体的近场和中场外,场的封闭形式格林函数在开发用于光学无源器件(如光学天线)的高效且严格的计算分析和设计工具方面也将发挥关键作用,这是通过显著提高积分方程数值解的计算成本来实现的。