Department of Biochemistry, Silesian Medical University, 41-808, Zabrze, Poland.
Biol Trace Elem Res. 2010 Jan;133(1):60-70. doi: 10.1007/s12011-009-8412-z. Epub 2009 Jun 2.
The intensified or uncontrolled formation of reactive oxygen species leads to disturbances of numerous biochemical processes. Among the factors inducing intensified free radical processes, fluoride ions are listed, among others. One of the organs most exposed to the toxic activity of fluorides is the kidney. In the study presented here, the influence of fluorine upon the activity of selected antioxidant enzymes in rat kidney has been examined, as well as antioxidant properties of methionine during intoxication with sodium fluoride. The experiment was carried out on Wistar FL rats (adult females) that for 35 days were administered water, NaF, NaF with methionine (doses: 10 mg NaF/kg bw/day, 10 mg Met/kg bw/day) . The influence of administered NaF and Met upon the antioxidative system in kidney was examined by analyzing the activity of the most important antioxidative enzymes (SOD, CAT, GPX, GR, GST). The studies carried out confirmed the disadvantageous effect of NaF upon the antioxidative system in rats (decrease in activity of antioxidative enzymes). Methionine increased the activity of antioxidative enzymes, most efficiently that of GPX, GR, and GST.
活性氧物质的大量形成或不受控制的形成会导致许多生化过程的紊乱。在诱导自由基过程加剧的因素中，氟化物离子也在其中。最容易受到氟化物毒性影响的器官之一是肾脏。在本研究中，研究了氟化物对大鼠肾脏中某些抗氧化酶活性的影响，以及蛋氨酸在氟化钠中毒时的抗氧化特性。实验在 Wistar FL 大鼠（成年雌性）上进行，这些大鼠在 35 天内给予水、NaF、NaF 与蛋氨酸（剂量：10mg NaF/kg bw/天，10mg Met/kg bw/天）。通过分析最重要的抗氧化酶（SOD、CAT、GPX、GR、GST）的活性，研究了给予的 NaF 和 Met 对肾脏抗氧化系统的影响。研究证实了 NaF 对大鼠抗氧化系统的不利影响（抗氧化酶活性下降）。蛋氨酸增加了抗氧化酶的活性，对 GPX、GR 和 GST 的活性增加最为有效。
