形成用于单分子分析的α-溶血素纳米孔。

Forming an alpha-hemolysin nanopore for single-molecule analysis.

作者信息

Jetha Nahid N, Wiggin Matthew, Marziali Andre

机构信息

Department of Physics and Astronomy, University of British Columbia, V6T 1Z1, Vancouver, BC, Canada.

出版信息

Methods Mol Biol. 2009;544:113-27. doi: 10.1007/978-1-59745-483-4_9.

DOI:10.1007/978-1-59745-483-4_9

PMID:19488697

Abstract

Nanopore analysis of single molecules can be performed by measuring the modulation in ionic current passing through the nanopore while an individual biomolecule such as DNA or RNA is resident in, translocating through, or otherwise interacting with the pore. The corresponding current signature has been shown to reveal properties of the biomolecule and information on its interactions with the pore. The alpha-hemolysin nanopore remains the pore of choice, particularly for single-molecule analysis of nucleic acids, because of its internal dimensions, hydrophilicity, and low-noise characteristics. In this chapter we present a detailed protocol for forming a robust alpha-hemolysin nanopore (or multiple nanopores) for single-molecule analysis.

摘要

通过测量当单个生物分子（如DNA或RNA）驻留在纳米孔中、穿过纳米孔或与纳米孔以其他方式相互作用时，通过纳米孔的离子电流调制，可进行单分子的纳米孔分析。已证明相应的电流特征能够揭示生物分子的特性及其与纳米孔相互作用的信息。α-溶血素纳米孔仍然是首选的纳米孔，特别是对于核酸的单分子分析，这是由于其内部尺寸、亲水性和低噪声特性。在本章中，我们将介绍一种用于形成用于单分子分析的坚固的α-溶血素纳米孔（或多个纳米孔）的详细方案。