Movileanu Liviu
Department of Physics, Syracuse University, 201 Physics Building, Syracuse, NY 13244-1130, USA.
Trends Biotechnol. 2009 Jun;27(6):333-41. doi: 10.1016/j.tibtech.2009.02.008. Epub 2009 Apr 23.
A single nanopore represents an amazingly versatile single-molecule probe that can be employed to reveal several important features of polypeptides, such as their folding state, backbone flexibility, mechanical stability, binding affinity to other interacting ligands and enzymatic activity. Moreover, groundwork in this area using engineered protein nanopores has demonstrated new opportunities for discovering the biophysical rules that govern the transport of proteins through transmembrane protein pores. In this review, I summarize the current knowledge in the field and discuss how nanopore probe techniques will provide a new generation of research tools in nanomedicine for quantitatively examining the details of complex recognition and, furthermore, will represent a crucial step in designing other pore-based nanostructures and high-throughput devices for molecular biomedical diagnosis.
单个纳米孔是一种极其通用的单分子探针，可用于揭示多肽的几个重要特征，如它们的折叠状态、主链柔韧性、机械稳定性、与其他相互作用配体的结合亲和力以及酶活性。此外，利用工程化蛋白质纳米孔在该领域的基础研究为发现控制蛋白质通过跨膜蛋白孔运输的生物物理规则带来了新机遇。在这篇综述中，我总结了该领域的现有知识，并讨论了纳米孔探针技术如何为纳米医学提供新一代研究工具，用于定量研究复杂识别的细节，此外，还将代表设计其他基于孔的纳米结构和用于分子生物医学诊断的高通量设备的关键一步。
