Heller William T, Littrell Kenneth C
Center for Structural Molecular Biology, Oak Ridge National Laboratory, Oak Ridge, TN, USA.
Methods Mol Biol. 2009;544:293-305. doi: 10.1007/978-1-59745-483-4_19.
As researchers strive to understand the interplay between the complex molecular systems that make up living cells, tools for characterizing the interactions between the various players involved have developed. Small-angle neutron scattering (SANS) plays an important role in building a molecular-level understanding of the structures of macromolecular systems that make up cells. SANS is widely applicable to the study of biological structures including, but by no means limited to, protein-protein or protein-nucleic acid complexes, lipid membranes, cellular scaffolding, and amyloid plaques. Here, we present a brief description of the technique as it is commonly applied to the study of biological systems and an overview instrumentation that is available at the various facilities around the world.
随着研究人员努力理解构成活细胞的复杂分子系统之间的相互作用，用于表征所涉及的各种分子之间相互作用的工具得到了发展。小角中子散射（SANS）在从分子水平理解构成细胞的大分子系统结构方面发挥着重要作用。SANS广泛应用于生物结构的研究，包括但不限于蛋白质-蛋白质或蛋白质-核酸复合物、脂质膜、细胞支架和淀粉样斑块。在此，我们简要介绍该技术在生物系统研究中的常用应用，并概述世界各地不同设施中可用的仪器设备。
