Georgiev Milen I, Weber Jost, Maciuk Alexandre
Department of Microbial Biosynthesis and Biotechnologies, Institute of Microbiology, Bulgarian Academy of Sciences, Plovdiv, Bulgaria.
Appl Microbiol Biotechnol. 2009 Jul;83(5):809-23. doi: 10.1007/s00253-009-2049-x. Epub 2009 Jun 2.
More than a century has passed since the first attempt to cultivate plant cells in vitro. During this time, plant cell cultures have become increasingly attractive and cost-effective alternatives to classical approaches for the mass production of plant-derived metabolites. Furthermore, plant cell culture is the only economically feasible way of producing some high-value metabolites (e.g., paclitaxel) from rare and/or threatened plants. This review summarizes recent advances in bioprocessing aspects of plant cell cultures, from callus culture to product formation, with particular emphasis on the development of suitable bioreactor configurations (e.g., disposable reactors) for plant cell culture-based processes; the optimization of bioreactor culture environments as a powerful means to improve yields; bioreactor operational modes (fed-batch, continuous, and perfusion); and biomonitoring approaches. Recent trends in downstream processing are also considered.
自首次尝试体外培养植物细胞以来，已经过去了一个多世纪。在此期间，植物细胞培养已成为大规模生产植物源代谢物的经典方法越来越有吸引力且更具成本效益的替代方法。此外，植物细胞培养是从珍稀和/或濒危植物中生产一些高价值代谢物（如紫杉醇）的唯一经济可行的方法。本综述总结了植物细胞培养生物加工方面的最新进展，从愈伤组织培养到产物形成，特别强调了用于基于植物细胞培养的工艺的合适生物反应器配置（如一次性反应器）的开发；作为提高产量的有力手段的生物反应器培养环境的优化；生物反应器操作模式（补料分批、连续和灌注）；以及生物监测方法。还考虑了下游加工的最新趋势。
