[利用表面增强激光解吸/电离飞行时间质谱法检测蕈样肉芽肿患者的组织蛋白生物标志物]
[Detection of tissue protein biomarkers by surface enhanced laser desorption/ionization-time of flight-mass spectrometry in patients with mycosis fungoides].
作者信息
Liu Jie, Zheng Yan-Hua, Zhou Yun-Fei, Zhang Jian-Zhong, Wang Bao-Xi
机构信息
Department of Dermatology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
出版信息
Zhonghua Yi Xue Za Zhi. 2009 Jan 13;89(2):100-4.
OBJECTIVE
To search the tissue biomarker in mycosis fungoides (MF) patients by surface enhanced laser desorption/ionization-time of flight-mass spectrometry (SELDI-TOF-MS).
METHODS
Skin specimens were collected from 12 MF patients and 15 normal controls to prepare solution of protein. CM10 Proteinchip was used to detect the biomarkers for MF. Differentially expressed protein was identified by immuno capturing.
RESULTS
The expression intensity levels of 24 proteins were different between the MF patients and controls. By searching in SWISS-PRO database and literature, the 3498 and 10 837 peaks were found to accord with HNP3 and S100A8 proteins respectively. By immuno capturing, 10 837 could be identified as S100A8 with a special antibody against S100A8.
CONCLUSION
A quick, easy, and high throughout proteome analytic method, SELDI-TOF-MS can identify the differential expressed proteins from MF tissue, thus offering a unique platform for biomarker detection of MF.
目的
通过表面增强激光解吸/电离飞行时间质谱（SELDI-TOF-MS）寻找蕈样肉芽肿（MF）患者的组织生物标志物。
方法
收集12例MF患者和15例正常对照的皮肤标本以制备蛋白质溶液。使用CM10蛋白质芯片检测MF的生物标志物。通过免疫捕获鉴定差异表达的蛋白质。
结果
MF患者和对照之间24种蛋白质的表达强度水平不同。通过在SWISS-PRO数据库和文献中搜索，发现3498和10837个峰分别与HNP3和S100A8蛋白一致。通过免疫捕获，使用抗S100A8的特异性抗体可将10837鉴定为S100A8。
结论
SELDI-TOF-MS是一种快速、简便且高通量的蛋白质组分析方法，可鉴定MF组织中差异表达的蛋白质，从而为MF的生物标志物检测提供独特的平台。
Zotero 插件