Institute of Biomedical Chemistry, Moscow, Russia.
Proteomics Clin Appl. 2011 Aug;5(7-8):432-9. doi: 10.1002/prca.201000165. Epub 2011 Jul 13.
The aim of this study was to estimate a possibility of mycosis fungoides (MF) diagnostics based on protein profiling in blood serum.
We obtained and analysed samples of blood serum from 23 patients with MF, and 29 psoriasis patients and 22 healthy donors as controls. Protein profiling was carried out using SELDI TOF MS SELDI-TOF and also profiling of 27 cytokines with multiplex immunoassay technology was implemented.
MS data analysis of sera did not give satisfactory statistical discrimination between the groups. Antibody-based cytokine profiling revealed a number of cytokines with a change in their concentrations in both MF and psoriasis (IL-1Ra, IL-4, G-CSF). The C-X-C motif chemokine 10 (IP-10, CXCL10) cytokine had a significantly increased concentration (p<0,001) in samples from MF patients as compared with the other groups.
IP-10 may be considered as a promising biomarker for the differentiation between MF and other skin conditions.
本研究旨在通过血清蛋白质谱分析来评估蕈样肉芽肿（MF）诊断的可能性。
我们收集并分析了 23 例 MF 患者、29 例银屑病患者和 22 例健康供者的血清样本。采用 SELDI-TOF MS（SELDI-TOF）进行蛋白质谱分析，同时采用多重免疫分析技术对 27 种细胞因子进行分析。
MS 数据分析未能在各组之间提供令人满意的统计学区分。基于抗体的细胞因子谱分析显示，MF 和银屑病患者的多种细胞因子浓度发生变化（IL-1Ra、IL-4、G-CSF）。与其他组相比，MF 患者血清中的 C-X-C 基序趋化因子 10（IP-10、CXCL10）细胞因子浓度显著升高（p<0.001）。
IP-10 可被视为区分 MF 和其他皮肤疾病的有前途的生物标志物。
