Tanaka Yoshiyuki, Hosokawa Munetaka, Otsu Keigo, Watanabe Tatsuo, Yazawa Susumu
Graduate School of Agriculture, Kyoto University, Kitashirakawa, Kyoto, Japan.
J Agric Food Chem. 2009 Jun 24;57(12):5407-12. doi: 10.1021/jf900634s.
Capsiconinoid is a group of nonpungent capsaicinoid analogues produced in Capsicum fruits, which we recently identified. Capsiconinoids have agonist activity for transient receptor potential vanilloid type 1 (TRPV1), which is reported to be a receptor for capsaicin. It is, therefore, important to screen cultivars containing high levels of capsiconinoid for their use as a vegetable or dietary supplement. This study describes the quantitative analysis of capsiconinoid content in fruits of 35 Capsicum cultivars: 18 cultivars of C. annuum, 7 of C. baccatum, 5 of C. chinense, 4 of C. frutescens, and 1 of C. pubescens. Using high-performance liquid chromatography (HPLC), we found that 10 cultivars contained capsiconinoids. Capsiconinoid Baccatum (CCB) (C. baccatum var. praetermissum) showed the highest capsiconinoid content (3314 microg/g DW) and Charapita (C. chinense) had the second highest content. The other 8 cultivars had much lower capsiconinoid content than these two cultivars (<300 microg/g DW). Time-course analysis during fruit development clarified that capsiconinoid content in CCB fruits increased until 30 days after flowering (DAF) and then decreased rapidly until 40 DAF.
辣椒素类物质是我们最近在辣椒果实中发现的一类无辣味的辣椒素类似物。辣椒素类物质对瞬时受体电位香草酸亚型1（TRPV1）具有激动剂活性，据报道TRPV1是辣椒素的受体。因此，筛选出辣椒素类物质含量高的品种用于蔬菜或膳食补充剂具有重要意义。本研究描述了对35个辣椒品种果实中辣椒素类物质含量的定量分析：18个一年生辣椒品种、7个浆果状辣椒品种、5个中国辣椒品种、4个灌木状辣椒品种和1个毛辣椒品种。使用高效液相色谱法（HPLC），我们发现10个品种含有辣椒素类物质。浆果状辣椒（CCB）（浆果状辣椒变种praetermissum）的辣椒素类物质含量最高（3314微克/克干重），查拉皮塔（中国辣椒）的含量次之。其他8个品种的辣椒素类物质含量远低于这两个品种（<300微克/克干重）。果实发育过程中的时间进程分析表明，CCB果实中的辣椒素类物质含量在开花后30天（DAF）前增加，然后在40 DAF前迅速下降。
