Chobe Leenata P, Lole Kavita S, Arankalle Vidya A
Hepatitis Division, National Institute of Virology, 20-A, Dr. Ambedkar Road, Pune 411001, Maharashtra, India.
Vet Microbiol. 2006 May 31;114(3-4):240-51. doi: 10.1016/j.vetmic.2005.12.007. Epub 2006 Feb 2.
The full-length genomic sequence of an Indian swine hepatitis E virus (HEV) isolate (IND-SW-00-01) recovered from feces of a pig experimentally infected with swine HEV pool from western India was determined. The genome consisted of 7,240 nucleotides, excluding the poly (A) tail of at least 22 residues and contained three open reading frames (ORFs), ORF-1 encoding 1,707 amino acids, ORF-2 encoding 674 amino acids and ORF-3 encoding 114 amino acids. Comparative full-length genome sequence and phylogenetic analyses suggested that the Indian swine HEV represents a distinct variant among the genotype 4 isolates with a divergence of 15-16.6%. Analyses based on ORF-1, 2 and 3 as well as partial ORF-2 (227 nucleotides) yielded similar results. As compared to type 4 HEV isolates, 26 unique amino acid substitutions were recorded, 16 in ORF-1, 8 in ORF-2 and 2 in ORF-3. IND-SW-00-01 showed insertion of 'C' at 5159 position while all other type 4 isolates have insertion of 'U' at the same position. Whether these changes contribute towards observed absence of type 4 HEV infections in Indian patients needs to be determined.
从一头用来自印度西部的猪戊型肝炎病毒（HEV）混合毒株进行实验性感染的猪粪便中分离出一株印度猪HEV毒株（IND-SW-00-01），并测定了其全长基因组序列。该基因组由7240个核苷酸组成，不包括至少22个残基的聚腺苷酸尾，包含三个开放阅读框（ORF），即编码1707个氨基酸的ORF-1、编码674个氨基酸的ORF-2和编码114个氨基酸的ORF-3。全长基因组序列比较和系统发育分析表明，印度猪HEV在4型分离株中代表一个独特的变异株，差异为15%-16.6%。基于ORF-1、2和3以及部分ORF-2（227个核苷酸）的分析得出了类似结果。与4型HEV分离株相比，记录到26个独特的氨基酸替换，其中16个在ORF-1中，8个在ORF-2中，2个在ORF-3中。IND-SW-00-01在5159位显示有“C”插入，而所有其他4型分离株在同一位置有“U”插入。这些变化是否导致印度患者中未观察到4型HEV感染，尚需确定。
