秋水仙碱特异性Fab片段对小鼠秋水仙碱毒性的逆转作用。

Reversal of murine colchicine toxicity by colchicine-specific Fab fragments.

作者信息

Urtizberea M, Sabouraud A, Cano N, Grandgeorge M, Rouzioux J M, Scherrmann J M

机构信息

INSERM U-26, Fernand Widal Hospital, Paris, France.

出版信息

Toxicol Lett. 1991 Oct;58(2):193-8. doi: 10.1016/0378-4274(91)90173-4.

DOI:10.1016/0378-4274(91)90173-4

PMID:1949077

Abstract

High-affinity Fab fragments (2 x 10(10) M-1) specific to colchicine were produced to evaluate their potency in reversing murine colchicine intoxication. Intraperitoneal injection of a 4.46 mg/kg colchicine dose was lethal for 100% of mice. 1.5 h after colchicine administration, a group of 10 mice was treated with colchicine-specific Fab fragments at a half-stoichiometrical dose compared to the colchicine dose by intravenous and intraperitoneal routes. 70% of the Fab-infused mice survived (P less than 0.01). This high efficiency of colchicine-specific Fab fragments in reversing acute murine colchicine toxicity suggests that Fab fragments would be an efficient antidote for the treatment of human colchicine poisoning.

摘要

制备了对秋水仙碱具有高亲和力的Fab片段（2×10¹⁰ M⁻¹），以评估其逆转小鼠秋水仙碱中毒的效力。腹腔注射4.46 mg/kg的秋水仙碱剂量对100%的小鼠是致命的。在给予秋水仙碱1.5小时后，一组10只小鼠通过静脉和腹腔途径接受与秋水仙碱剂量呈半化学计量比的秋水仙碱特异性Fab片段治疗。70%接受Fab片段注射的小鼠存活下来（P<0.01）。秋水仙碱特异性Fab片段在逆转急性小鼠秋水仙碱毒性方面的这种高效性表明，Fab片段将是治疗人类秋水仙碱中毒的有效解毒剂。