Krumnow April A, Sorokulova Iryna B, Olsen Eric, Globa Ludmila, Barbaree James M, Vodyanoy Vitaly J
Department of Anatomy, Physiology, and Pharmacology, Auburn University, Auburn, AL 36849, United States.
J Microbiol Methods. 2009 Aug;78(2):189-94. doi: 10.1016/j.mimet.2009.05.017. Epub 2009 May 30.
A new inexpensive and simple method for preserving microorganisms has been developed. Natural polymers of acacia gum and pullulan were used to preserve model bacteria Escherichia coli and Bacillus subtilis via immobilization and storage under various conditions. Formulation of E. coli and B. subtilis in acacia gum significantly increased the viability of both cultures during desiccation at 40 degrees C as well as during the storage at various temperatures and relative humidity. In the ranges of temperatures and humidity used in experiments, the high humidity affected the viability of E. coli more than high temperature. Thermodynamic parameters for E. coli thermal degradation were used for quantification of results and characterization of the preservation process. Viability of B. subtilis in acacia gum polymer was not significantly changed during the storage in the temperature and humidity experiments. The number of viable B. subtilis recovered after storage in pullulan, and in PBS under various humidity conditions was 1-2 logs less in comparison with the number of cells before storage. It was found that acacia gum provides better protection than pullulan for both bacteria during the preservation process.
一种用于保存微生物的新型廉价且简单的方法已被开发出来。阿拉伯胶和普鲁兰多糖等天然聚合物被用于通过固定化以及在各种条件下储存来保存模式细菌大肠杆菌和枯草芽孢杆菌。将大肠杆菌和枯草芽孢杆菌配制在阿拉伯胶中，显著提高了两种培养物在40摄氏度干燥过程以及在不同温度和相对湿度下储存期间的活力。在实验所使用的温度和湿度范围内，高湿度对大肠杆菌活力的影响比高温更大。利用大肠杆菌热降解的热力学参数对结果进行量化并表征保存过程。在温度和湿度实验储存期间，枯草芽孢杆菌在阿拉伯胶聚合物中的活力没有显著变化。与储存前的细胞数量相比，在不同湿度条件下储存在普鲁兰多糖和磷酸盐缓冲盐溶液（PBS）中的枯草芽孢杆菌的存活数量少1至2个对数级。研究发现，在保存过程中，阿拉伯胶对两种细菌的保护作用比普鲁兰多糖更好。
