Burlacu E, David C, Vasilescu T
Arch Roum Pathol Exp Microbiol. 1989 Jan-Mar;48(1):65-78.
The paper presents results obtained for the bacterial cultures preservation (E. coli ATCC 25 922 and S. aureus Wood) by three in vacuo desiccation procedures: freeze-drying (lyophilization or cryo-desiccation at eutectic zone), cryo-desiccation above eutectic zone and direct drying. It has been in view: the survival of liquid cultures as reported to the desiccation procedure per se, the loss of viability of desiccated cultures stored in refrigerator for at least one year and the residual moisture of desiccated cultures. For 3 batches there has been applied the accelerated thermal degradation test. Employing the same protective medium for both cultures, E. coli cultures prove to be more easily affected by lyophilization and cryo-desiccation above eutectic zone as compared to S. aureus cultures, fact that may be due to the differences between the wall structures of G--bacteria and G+ bacteria, respectively. During storage, E. coli and S. aureus cultures proved a quite similar loss of viability. The residual moisture content was quite similar for both E. coli and S. aureus cultures exposed to the same in vacuo desiccation procedure. The lyophilization and the cryo-desiccation above eutectic zone, as compared to direct drying, yielded superior results. The accelerated thermal degradation test provides only informative results, partially confirmed by viable counts determined at stated intervals of storage time in the refrigerator.
本文介绍了通过三种真空干燥程序（冷冻干燥（冻干或共晶区低温干燥）、共晶区以上低温干燥和直接干燥）对细菌培养物（大肠杆菌ATCC 25922和金黄色葡萄球菌Wood）进行保存的结果。研究考虑了以下方面：液体培养物在每种干燥程序下的存活率、在冰箱中储存至少一年的干燥培养物的活力丧失情况以及干燥培养物的残留水分。对3批样品进行了加速热降解试验。两种培养物使用相同的保护培养基，结果表明，与金黄色葡萄球菌培养物相比，大肠杆菌培养物更容易受到冻干和共晶区以上低温干燥的影响，这一情况可能分别归因于革兰氏阴性菌和革兰氏阳性菌细胞壁结构的差异。在储存期间，大肠杆菌和金黄色葡萄球菌培养物的活力丧失情况相当相似。对于采用相同真空干燥程序的大肠杆菌和金黄色葡萄球菌培养物，其残留水分含量相当相似。与直接干燥相比，冻干和共晶区以上低温干燥产生了更好的结果。加速热降解试验仅提供参考性结果，在冰箱中按规定储存时间间隔进行的活菌计数部分证实了这些结果。
