急性心肌梗死再灌注的闭胸实验猪模型

Closed-chest experimental porcine model of acute myocardial infarction-reperfusion.

作者信息

Pérez de Prado Armando, Cuellas-Ramón Carlos, Regueiro-Purriños Marta, Gonzalo-Orden J Manuel, Pérez-Martínez Claudia, Altónaga José R, García-Iglesias M José, Orden-Recio M Asunción, García-Marín Juan F, Fernández-Vázquez Felipe

机构信息

Section of Interventional Cardiology, Cardiology Service, Hospital de León, Spain.

出版信息

J Pharmacol Toxicol Methods. 2009 Nov-Dec;60(3):301-6. doi: 10.1016/j.vascn.2009.05.007. Epub 2009 May 31.

DOI:10.1016/j.vascn.2009.05.007

PMID:19490947

Abstract

INTRODUCTION

Progress in cardiovascular regenerative medicine research requires the availability of appropriate experimental animal models that are as close to humans as feasible. Our objective was to assess the validity of a porcine endovascular model of myocardial infarction and reperfusion.

METHODS

Fifteen domestic pigs (Large White race) were anesthetized and pre-medicated with amiodarone. Endovascular fluoroscopy-guided coronary procedures were performed to occlude the mid-left anterior descending artery using a coronary angioplasty balloon. Occlusion was confirmed by angiography and electrocardiography. After 75 min the balloon catheter system was withdrawn and the presence of reperfusion flow was verified. The animals were sacrificed after 1 and 2 weeks of follow-up, the hearts were explanted, and the extent of myocardial infarction with respect to the left ventricle was quantified.

RESULTS

Overall survival rate was 67%. Five animals died prematurely: 3 showing signs of heart failure, 1 had reperfusion failure (final TIMI flow grade 1) and 1 succumbed to acute stress. The most common adverse event was ventricular fibrillation (87% of the animals) and defibrillation was effective in all affected animals. The extent of myocardial infarct in the animals followed-up for 1 and 2 weeks was similar (20.4+/-4.3% vs. 20.9+/-2.8%, respectively; p=0.8) but was significantly greater in the animals that died prematurely (29.5+/-3.6%, p=0.02).

CONCLUSIONS

The endovascular porcine model we have explored constitutes a feasible and reproducible alternative for the evaluation of human myocardial infarction and reperfusion.

摘要

引言

心血管再生医学研究的进展需要有尽可能接近人类的合适实验动物模型。我们的目的是评估猪心肌梗死和再灌注血管内模型的有效性。

方法

15头家猪（大白品种）麻醉后预先给予胺碘酮。在血管内荧光透视引导下进行冠状动脉操作，使用冠状动脉成形术球囊闭塞左前降支中段。通过血管造影和心电图确认闭塞。75分钟后撤出球囊导管系统，验证再灌注血流的存在。随访1周和2周后处死动物，取出心脏，量化左心室心肌梗死范围。

结果

总生存率为67%。5只动物过早死亡：3只出现心力衰竭迹象，1只出现再灌注失败（最终TIMI血流分级为1级），1只因急性应激死亡。最常见的不良事件是室颤（87%的动物），所有受影响动物的除颤均有效。随访1周和2周的动物心肌梗死范围相似（分别为20.4±4.3%和20.9±2.8%；p=0.8），但过早死亡的动物心肌梗死范围明显更大（29.5±3.6%，p=0.02）。