Crouch A A, Seow W K, Whitman L M, Smith S E, Thong Y H
Department of Child Health, University of Queensland, Mater Children's Hospital, South Brisbane, Australia.
Trans R Soc Trop Med Hyg. 1991 May-Jun;85(3):375-9. doi: 10.1016/0035-9203(91)90297-c.
Human neutrophils and monocytes were found to be capable of interfering with the in vitro adherence of Giardia intestinalis. Significantly greater inhibition of adherence was shown by stimulated phagocytic cells than by unstimulated cells. Both azurophil and specific granules extracted from the cytoplasm of neutrophils were equally potent in their adherence inhibitory activity. Dose-dependent effects on adherence were observed with granular enzymes and reactive oxygen species. Lower concentrations of reactive oxygen species were required for adherence inhibition that for growth inhibition. These results suggest that the adherence mechanism of G. intestinalis may be a feasible target for immunological attack by phagocytic cells.
研究发现，人类中性粒细胞和单核细胞能够干扰小肠贾第虫的体外黏附。与未受刺激的细胞相比，受刺激的吞噬细胞对黏附的抑制作用显著更强。从嗜中性粒细胞胞质中提取的嗜天青颗粒和特异性颗粒，其黏附抑制活性相当。观察到颗粒酶和活性氧物质对黏附具有剂量依赖性效应。与生长抑制相比，抑制黏附所需的活性氧物质浓度更低。这些结果表明，小肠贾第虫的黏附机制可能是吞噬细胞进行免疫攻击的一个可行靶点。
