Kelly M K, Thong Y H
J Immunol Methods. 1984 Nov 30;74(2):345-51. doi: 10.1016/0022-1759(84)90302-8.
A simple rapid method for the measurement of human monocyte adherence using nylon fibre microcolumns is described. The kinetics indicate the optimal contact time to be 30 min for monocytes, compared with 10 min for neutrophils. The optimal temperature is 37 degrees C; significantly low values were obtained for 4 degrees C and 45 degrees C, while intermediate values were obtained for 25 degrees C. Monocyte adherence was more sensitive to inhibition by fluoride than cyanide, suggesting that energy for adherence is mainly derived from the glycolytic pathway. The addition of phorbol myristate acetate enhances monocyte adherence. Significant decay in monocyte adherence occurred after isolation from whole blood for 24 h or longer.
本文描述了一种使用尼龙纤维微柱测量人单核细胞黏附的简单快速方法。动力学表明，单核细胞的最佳接触时间为30分钟，而中性粒细胞为10分钟。最佳温度为37摄氏度；4摄氏度和45摄氏度时得到的值显著较低，而25摄氏度时得到的值处于中间水平。单核细胞黏附对氟化物抑制比氰化物更敏感，这表明黏附所需能量主要来自糖酵解途径。添加佛波酯肉豆蔻酸酯可增强单核细胞黏附。从全血中分离24小时或更长时间后，单核细胞黏附出现显著下降。
