Fabian M A, Bollinger R R, Wyble C W, Watkins W D, Canada A T
Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710.
Transplantation. 1991 Nov;52(5):794-9. doi: 10.1097/00007890-199111000-00007.
A number of organ preservation solutions have been formulated to slow the inevitable progression of ischemic injury, thus prolonging the storage time between removal and implantation. As adenine nucleotide content has been shown to correlate with the functional recovery of transplanted livers and hearts, this study investigated the effects of 24 hr of storage in three preservation solutions, saline (SA), Euro-Collins (CO), and University of Wisconsin (UW) on adenine and nicotinamide adenine nucleotides and inosine content of the rat small intestine. Significant biochemical differences were found between segments as early as after the initial perfusion when the inosine content was higher in UW-perfused than CO- or SA-perfused segments. After 2 hr of storage in CO solution and after 6 and 24 hr in both CO and UW solutions, the ATP content was higher than in SA-stored segments. In addition to inosine, which was significantly higher at all time points for UW-stored segments, the AMP and total adenine nucleotide content of UW-stored segments at 24 hr was significantly higher than SA- or CO-stored segments. After 24 hr of storage, those segments stored in UW were able to utilize significantly more oxygen than SA-stored. These data provide biochemical evidence supporting the advantages of CO and UW storage solutions over SA for preservation of small intestine segments.
人们已经配制了多种器官保存溶液，以减缓缺血性损伤不可避免的进展，从而延长器官切除与植入之间的保存时间。由于腺嘌呤核苷酸含量已被证明与移植肝脏和心脏的功能恢复相关，本研究调查了在三种保存溶液（生理盐水（SA）、欧洲柯林斯溶液（CO）和威斯康星大学溶液（UW））中储存24小时对大鼠小肠腺嘌呤、烟酰胺腺嘌呤核苷酸和肌苷含量的影响。早在初始灌注后，各段之间就发现了显著的生化差异，此时UW灌注段的肌苷含量高于CO或SA灌注段。在CO溶液中储存2小时后，以及在CO和UW溶液中分别储存6小时和24小时后，ATP含量高于SA储存段。除了UW储存段在所有时间点的肌苷含量显著更高外，UW储存段在24小时时的AMP和总腺嘌呤核苷酸含量也显著高于SA或CO储存段。储存24小时后，UW储存的段比SA储存的段能够消耗显著更多的氧气。这些数据提供了生化证据，支持CO和UW保存溶液在保存小肠段方面优于SA。
