Palombo J D, Pomposelli J J, Fechner K D, Blackburn G L, Bistrian B R
Department of Medicine, New England Deaconess Hospital/Harvard Medical School, Boston, Massachusetts 02215.
Transplantation. 1991 Apr;51(4):867-73. doi: 10.1097/00007890-199104000-00025.
The extensive reduction of adenine nucleotides during preservation coupled with the loss of salvageable precursors during initial reflow may exacerbate recovery of adenine nucleotides in allograft liver following transplantation. The objective of this study was to assess whether provision of adenosine during reperfusion of rat liver stored for 20 hr in University of Wisconsin solution could enhance adenine nucleotide restoration. ATP and total adenine nucleotide content of livers perfused with an oxygenated Krebs/fluorocarbon solution containing 1 mM adenosine were restored to levels in vivo within 30 min of perfusion. Adenine nucleotide recovery in livers perfused without adenosine was only 65% of normal. Acute nutritional deprivation of the donor rats had no effect on adenine nucleotide restoration. These results indicate that a conditional deficiency of intracellular nucleotide precursors exists during initial reperfusion of liver subjected to extended storage in UW solution. Provision of supplemental adenosine to the allograft liver during initial reflow appears warranted to promote full and rapid restoration of adenine nucleotide content following extended preservation ex vivo.
保存期间腺嘌呤核苷酸大量减少，再加上初始复流期间可挽救前体的丧失，可能会加剧移植后同种异体移植肝脏中腺嘌呤核苷酸的恢复。本研究的目的是评估在威斯康星大学溶液中储存20小时的大鼠肝脏再灌注期间提供腺苷是否能增强腺嘌呤核苷酸的恢复。用含有1 mM腺苷的含氧Krebs/氟碳溶液灌注的肝脏，其ATP和总腺嘌呤核苷酸含量在灌注后30分钟内恢复到体内水平。未用腺苷灌注的肝脏中腺嘌呤核苷酸的恢复仅为正常水平的65%。供体大鼠的急性营养剥夺对腺嘌呤核苷酸的恢复没有影响。这些结果表明，在UW溶液中长时间储存的肝脏初始复流期间，存在细胞内核苷酸前体的条件性缺乏。在初始复流期间向同种异体移植肝脏提供补充腺苷似乎有助于促进长时间体外保存后腺嘌呤核苷酸含量的完全和快速恢复。
