Ianovich V G, Vovk S I, Brodin S V
Ukr Biokhim Zh (1978). 1991 Jul-Aug;63(4):56-61.
In the experiments involving incubation of the liver, brain cortex, muscle and adipose tissues homogenates with [3-14C] tryptophan for an hour 43.2-89.3% of the label was found in proteins, 7.2-47.2%--in lipids, 2.6-9.4%--in CO2. Following incubation of the above-mentioned tissue homogenates with [2-14C] alanine, proteins, lipids and CO2 contain 28.8-49.3%; 22.6-31.9% and 21.6-49.3% of radioactive label, respectively. Radioactivity of lipids synthesized by the homogenates of the investigated tissues from [3-14C] tryptophan and [2-14C] alanine is 23.5-63.5 and 21.1-56.0%, respectively, the radioactivity of CO2 being 1.4-5.1 and 9.3-11.8% of the above-mentioned compounds synthesized from [1-14C] acetate. The results obtained testify to the considerable contribution of [3-14C] tryptophan and [2-14C] alanine to protein synthesis as well as to their involvement in the substrate supply of lipogenesis and energetic processes in various organs and tissues of cattle.
在涉及将肝脏、大脑皮层、肌肉和脂肪组织匀浆与[3-14C]色氨酸孵育一小时的实验中，发现43.2-89.3%的放射性标记存在于蛋白质中，7.2-47.2%存在于脂质中，2.6-9.4%存在于二氧化碳中。在将上述组织匀浆与[2-14C]丙氨酸孵育后，蛋白质、脂质和二氧化碳分别含有28.8-49.3%、22.6-31.9%和21.6-49.3%的放射性标记。由被研究组织匀浆从[3-14C]色氨酸和[2-14C]丙氨酸合成的脂质的放射性分别为23.5-63.5%和21.1-56.0%，二氧化碳的放射性分别为从[1-14C]乙酸合成的上述化合物的1.4-5.1%和9.3-11.8%。所得结果证明[3-14C]色氨酸和[2-14C]丙氨酸对蛋白质合成有相当大的贡献，并且它们参与了牛的各种器官和组织中脂肪生成和能量过程的底物供应。
