Tsutsumi Moe, Ikeyama Kazuyuki, Denda Sumiko, Nakanishi Jotaro, Fuziwara Shigeyoshi, Aoki Hirofumi, Denda Mitsuhiro
Shiseido Research Center, Fukuura, Kanazawa-ku, Yokohama, Japan.
Exp Dermatol. 2009 Jun;18(6):567-70. doi: 10.1111/j.1600-0625.2009.00851.x.
Previous reports have suggested the existence of photoreceptors for visible radiation at the surface of the human body. Rhodopsin is a well-known photosensitive protein found in the rod cells of the retina and detects light/dark contrast. Cone opsins are also photosensitive receptors in the cone cells of the retina and detect colour. Here, we describe immunochemical studies using anti-rhodopsin and anti-opsin antibodies on human skin. Both mouse retina and human epidermis showed clear immunoreactivity with each antibody. Interestingly, immunoreactivity against longer-wavelength opsin antibody was observed in the basal layer of the epidermis, while immunoreactivity against rhodopsin and shorter-wavelength opsin was observed in the upper layer. PCR analysis confirmed the expression of rhodopsin-like and opsin-like genes in human retina and the skin. These results suggest that a series of proteins, which play a crucial role in visual perception, are expressed in human epidermis.
先前的报告表明人体表面存在对可见光辐射的光感受器。视紫红质是一种在视网膜视杆细胞中发现的著名光敏蛋白，可检测明暗对比度。视锥视蛋白也是视网膜视锥细胞中的光敏受体，可检测颜色。在此，我们描述了使用抗视紫红质和抗视蛋白抗体对人体皮肤进行的免疫化学研究。小鼠视网膜和人表皮对每种抗体均显示出明显的免疫反应性。有趣的是，在表皮基底层观察到针对长波长视蛋白抗体的免疫反应性，而在上层观察到针对视紫红质和短波长视蛋白的免疫反应性。PCR分析证实视紫红质样和视蛋白样基因在人视网膜和皮肤中表达。这些结果表明，一系列在视觉感知中起关键作用的蛋白质在人表皮中表达。
