VR1在人皮肤表皮角质形成细胞上的免疫反应性。

Immunoreactivity of VR1 on epidermal keratinocyte of human skin.

作者信息

Denda M, Fuziwara S, Inoue K, Denda S, Akamatsu H, Tomitaka A, Matsunaga K

机构信息

Shiseido Life Science Research Center, 2-12-1 Fukuura, Kanazawa-ku, Yokohama, 236-8643, Japan.

出版信息

Biochem Biophys Res Commun. 2001 Aug 3;285(5):1250-2. doi: 10.1006/bbrc.2001.5299.

DOI:10.1006/bbrc.2001.5299

PMID:11478791

Abstract

We demonstrated the immunoreactivity of the receptor proteins, VR1, ion channels associated with pain sensation, on the epidermis of the human skin. Immunohistochemistry using antiserum against VR1 derived peptide showed immunoreactivity on the keratinocytes cell membrane of the human epidermis and cultured keratinocytes. The blocking peptide of the antiserum reduced the immunoreactivity on the epidermis. RT-PCR assay of cultured human keratinocyte also showed expression of VR1 mRNA. These results suggest the existence of VR1-like protein in epidermal keratinocytes of human skin.

摘要

我们证明了与疼痛感觉相关的受体蛋白VR1在人皮肤表皮上的免疫反应性。使用抗VR1衍生肽的抗血清进行免疫组织化学显示，在人表皮的角质形成细胞膜和培养的角质形成细胞上有免疫反应性。抗血清的封闭肽降低了表皮上的免疫反应性。对培养的人角质形成细胞进行的RT-PCR分析也显示了VR1 mRNA的表达。这些结果表明在人皮肤的表皮角质形成细胞中存在VR1样蛋白。