Wang Liang, Shen Tong, Zhou Cheng-fan, Yu Jun-feng, Zhu Qi-xing
Department of Occupational and Environmental Health, School of Public Health, Anhui Medical University, Hefei 230032, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2009 Apr;27(4):207-10.
To study the changes of nitric oxide (NO) in the BALB/c hairless mice skin after trichloroethylene (TCE) irritation and the protection of ginkgo biloba extract (GbE) and vitamin E (VE).
132 BALB/c hairless mice were randomly divided into blank control group, solvent group (olive oil), TCE groups (20%TCE, 40%TCE, 80%TCE and 100%TCE), GbE groups (0.1%GbE, 1%GbE and 10%GbE) and VE groups (5%VE, 10% VE and 20% VE), with 11 animals in each group, 5 for acute irritation test and 6 for the cumulative irritation test. The skin irritation was observed, and the levels of NO in the dorsal skin of BALB/C hairless mice were detected. The kit of NO was used to detect the levels of NO in the dorsal skin of BALB/c hairless mice.
(1) The skin presented erythema and edema after TCE irritation both in acute irritation and cumulative irritation test and the skin inflammation showed time-dose effect relationship; the mice skin was protected in GbE or VE groups. (2) In the acute stimulation test, the levels of NO in 80%TCE group (69.895 +/- 9.605 micromol/mg pro) and 100%TCE group (77.273 +/- 9.290 micromol/mg pro) were significantly different compared with blank control group and solvent control group (P < 0.05 or P < 0.01). In the protection group, the NO level were reduced, with the statistically significant differences. (3) In acute irritation test, the levels of NO in 80%TCE group (60.362 +/- 9.817 micromol/mg pro) and 100%TCE group (68.027 +/- 9.354 micromol/mg pro) were significantly different compared with blank control group and solvent control group, (P < 0.05 or P < 0.01); In the protection group, 1% GbE, 10% GbE, 10% VE and 20%VE could reduce the levels of NO, with statistically significant differences.
TCE can produce the irritation on the dorsal skin of BALB/c hairless mice and induce the significant increase of the NO levels. GbE and VE can protect the skin from TCE irritation damage.
研究三氯乙烯（TCE）刺激后BALB/c无毛小鼠皮肤中一氧化氮（NO）的变化以及银杏叶提取物（GbE）和维生素E（VE）的保护作用。
将132只BALB/c无毛小鼠随机分为空白对照组、溶剂组（橄榄油）、TCE组（20%TCE、40%TCE、80%TCE和100%TCE）、GbE组（0.1%GbE、1%GbE和10%GbE）和VE组（5%VE、10%VE和20%VE），每组11只，其中5只用于急性刺激试验，6只用于累积刺激试验。观察皮肤刺激情况，并检测BALB/C无毛小鼠背部皮肤中NO的水平。采用NO试剂盒检测BALB/c无毛小鼠背部皮肤中NO的水平。
（1）在急性刺激试验和累积刺激试验中，TCE刺激后皮肤均出现红斑和水肿，且皮肤炎症呈时间-剂量效应关系；GbE或VE组对小鼠皮肤有保护作用。（2）在急性刺激试验中，80%TCE组（69.895±9.605微摩尔/毫克蛋白）和100%TCE组（77.273±9.290微摩尔/毫克蛋白）的NO水平与空白对照组和溶剂对照组相比有显著差异（P<0.05或P<0.01）。在保护组中，NO水平降低，差异有统计学意义。（3）在急性刺激试验中，80%TCE组（60.362±9.817微摩尔/毫克蛋白）和100%TCE组（68.027±9.354微摩尔/毫克蛋白）的NO水平与空白对照组和溶剂对照组相比有显著差异（P<0.05或P<0.01）；在保护组中，1%GbE、10%GbE、10%VE和20%VE可降低NO水平，差异有统计学意义。
TCE可对BALB/c无毛小鼠背部皮肤产生刺激，并导致NO水平显著升高。GbE和VE可保护皮肤免受TCE刺激损伤。
