Yu Jun-feng, Zhu Qi-xing
Department of Occupational Health, School of Public Health, Anhui Medical University, Hefei 230032, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2009 Jan;27(1):16-20.
To investigate the expression of apoptosis and caspase-8, cyt c in the skin of the BALB/c mice exposed to trichloroethylene (TCE).
30 BALB/c mice were divided in random into the solvent control group, 10% TCE group, 20% TCE group, 40% TCE group, 80% TCE group and 100% TCE group. Apoptotic cells were detected by TUNEL and EM. The expressions of caspase-8 and cyt c were detected with immunohistochemical method.
EM showed that the apoptosis of cells was found in the high dosage groups. The immunohistochemical results showed that there were significant differences in the apoptosis rate and the activity of cyt c between the different dosage groups. There was the significant difference in the apoptosis rate between the 40%, 80%, 100% TCE groups the control group (P < 0.01). There was the significant difference in the expression of cyt c between the 20%, 40%, 80%, 100% TCE groups [(2.60 +/- 0.54), (3.42 +/- 0.56), (5.81 +/- 1.30) and (6.00 +/- 0.70), respectively] and the control group (P < 0.01). The expressions of caspase-8 had no significant differences (P > 0.05).
Apoptosis plays an important role in trichloroethylene induced irritant injury in skin and the apoptosis may be related with the mitochondrial injury.
探讨三氯乙烯（TCE）染毒的BALB/c小鼠皮肤中细胞凋亡及半胱天冬酶-8（caspase-8）、细胞色素c（cyt c）的表达情况。
将30只BALB/c小鼠随机分为溶剂对照组、10% TCE组、20% TCE组、40% TCE组、80% TCE组和100% TCE组。采用脱氧核糖核苷酸末端转移酶介导的缺口末端标记法（TUNEL）和电镜检测凋亡细胞。用免疫组织化学方法检测caspase-8和cyt c的表达。
电镜显示高剂量组出现细胞凋亡。免疫组织化学结果显示，不同剂量组之间凋亡率和cyt c活性存在显著差异。40%、80%、100% TCE组与对照组之间凋亡率有显著差异（P < 0.01）。20%、40%、80%、100% TCE组[分别为(2.60±0.54)、(3.42±0.56)、(5.81±1.30)和(6.00±0.70)]与对照组之间cyt c表达有显著差异（P < 0.01）。caspase-8的表达无显著差异（P > 0.05）。
细胞凋亡在三氯乙烯所致皮肤刺激性损伤中起重要作用，且细胞凋亡可能与线粒体损伤有关。
