Koshida K, Uchibayashi T, Hisazumi H
Department of Urology, Kanazawa University, Japan.
Urol Int. 1991;47 Suppl 1:96-9. doi: 10.1159/000282263.
Characteristics of placental-like alkaline phosphatase (PLAP-like enzyme) in seminoma was studied. By use of lectin affinity chromatography, PLAP-like enzyme in seminoma revealed extra sugar chains compared to placental alkaline phosphatase (PLAP), indicating heterogeneity of the carbohydrate moiety. However, the glycosylation patterns were found to be essentially similar between seminoma and normal testis. On isoelectric focusing, differences in migration patterns were revealed between seminoma-derived and normal testis-derived PLAP-like enzyme as well as between PLAP-like enzyme and PLAP. The differences in charge were mainly due to differences in sialylation of the molecules. The complex pattern of PLAP-like enzyme from seminoma on isoelectric focusing was not altered by neuraminidase treatment, indicating a considerable charge heterogeneity within the population of the enzyme molecules from the tumor.
对精原细胞瘤中胎盘样碱性磷酸酶（PLAP样酶）的特性进行了研究。通过凝集素亲和层析法，与胎盘碱性磷酸酶（PLAP）相比，精原细胞瘤中的PLAP样酶显示出额外的糖链，表明碳水化合物部分存在异质性。然而，发现精原细胞瘤和正常睾丸之间的糖基化模式基本相似。在等电聚焦时，精原细胞瘤来源的和正常睾丸来源的PLAP样酶之间以及PLAP样酶和PLAP之间显示出迁移模式的差异。电荷差异主要是由于分子唾液酸化的差异。来自精原细胞瘤的PLAP样酶在等电聚焦上的复杂模式不受神经氨酸酶处理的影响，表明肿瘤中酶分子群体内存在相当大的电荷异质性。
Zotero 插件