Koshida K, Wahren B E
Department of Virology, National Bacteriological Laboratory, Stockholm, Sweden.
Clin Chim Acta. 1990 Jan 15;186(2):255-64. doi: 10.1016/0009-8981(90)90043-r.
Heterogeneity of placental-like alkaline phosphatase (PLAP-like enzyme) in seminoma was studied. PLAP-like enzyme from seminoma tissues was separated into three areas with different proportions between tumors, while PLAP and PLAP-like enzymes in normal testes were separated into two areas on the basis of hydrophobicity. By use of lectin affinity chromatography, PLAP-like enzyme in seminoma revealed extra sugar chains compared to PLAP, indicating heterogeneity of the carbohydrate moiety. However, the glycosylation patterns were found to be essentially similar between seminoma and normal testis. On isoelectric focusing, differences in migration patterns of PLAP-like enzyme were revealed between seminoma and normal testis as well as between PLAP-like enzyme and PLAP. The differences in charge were mainly due to differences in sialylation of the molecules. The complex pattern on isoelectric focusing was not altered by neuraminidase treatment, indicating a considerable charge heterogeneity within the population of PLAP-like enzyme molecules from seminoma.
研究了精原细胞瘤中胎盘样碱性磷酸酶（PLAP样酶）的异质性。来自精原细胞瘤组织的PLAP样酶在肿瘤之间按不同比例被分离为三个区域，而正常睾丸中的PLAP和PLAP样酶基于疏水性被分离为两个区域。通过凝集素亲和层析，与PLAP相比，精原细胞瘤中的PLAP样酶显示出额外的糖链，表明碳水化合物部分存在异质性。然而，发现精原细胞瘤和正常睾丸之间的糖基化模式基本相似。在等电聚焦时，精原细胞瘤与正常睾丸之间以及PLAP样酶与PLAP之间的PLAP样酶迁移模式存在差异。电荷差异主要是由于分子唾液酸化的差异。等电聚焦上的复杂模式未被神经氨酸酶处理改变，表明来自精原细胞瘤的PLAP样酶分子群体内存在相当大的电荷异质性。
