肯尼亚霍马湾人类免疫缺陷病毒感染率高的地区应用薄层琼脂法诊断涂片阴性肺结核的情况
Implementation of the thin layer agar method for diagnosis of smear-negative pulmonary tuberculosis in a setting with a high prevalence of human immunodeficiency virus infection in Homa Bay, Kenya.
作者信息
Martin Anandi, Munga Waweru Peter, Babu Okatch Fred, Amondi Ouma Naureen, Bonte Laurence, Varaine Francis, Portaels Françoise
机构信息
Institute of Tropical Medicine, Antwerp, Belgium.
出版信息
J Clin Microbiol. 2009 Aug;47(8):2632-4. doi: 10.1128/JCM.00264-09. Epub 2009 Jun 3.
Abstract
The objective of this study was to evaluate the performance of a low-cost method, the thin layer agar (TLA) method, for the diagnosis of smear-negative patients. This prospective study was performed in Homa Bay District Hospital in Kenya. Out of 1,584 smear-negative sputum samples, 212 (13.5%) were positive by culture in Löwenstein-Jensen medium (LJ) and 220 (14%) were positive by the TLA method. The sensitivities of LJ and TLA were 71% and 74%, respectively. TLA could become an affordable method for the diagnosis of smear-negative tuberculosis in resource-limited settings, with results available within 2 weeks.
摘要
本研究的目的是评估一种低成本方法——薄层琼脂（TLA）法在诊断涂片阴性患者中的性能。这项前瞻性研究在肯尼亚霍马湾地区医院进行。在1584份涂片阴性的痰标本中，212份（13.5%）在罗氏培养基（LJ）上培养呈阳性，220份（14%）通过TLA法检测呈阳性。LJ和TLA的敏感性分别为71%和74%。TLA可能成为资源有限地区诊断涂片阴性结核病的一种经济实惠的方法，且在2周内可获得结果。
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