Wills R B, Shaw C G, Day W R
J Chromatogr Sci. 1977 Jul;15(7):262-6. doi: 10.1093/chromsci/15.7.262.
Resolution of the water-soluble vitamins--pyridoxin, riboflavin, niacinamide, vitamin B12, thiamin, ascorbic acid, niacin and folic acid--by high pressure liquid chromatography was examined on two bonded-phase columns, muBondapak C18 and muBondapak NH2. The effect on the retention times of individual vitamins and the separation of a multivitamin sample was determined using varying proportions of water/methanol as the eluting solvent and by addition of various salts, buffer solutions and PIC reagents to the water/methanol. Each vitamin was able to be eluted satisfactorily from muBondapak C18. It was found that seven vitamins could be resolved from a multivitamin mixture in a single analysis in several solvent systems with the total time for the analyses being always less than 40 min. With muBondapak NH2, all the vitamins except folic acid were eluted and six vitamins could be resolved from a mixture in a single analysis. The speed of analysis was greater with muBondapak NH2 with all compounds eluted in 15 min and the peaks were sharper. The order of elution was essentially the reverse of that obtained with muBondapak C18.
在两根键合相柱（μBondapak C18和μBondapak NH2）上，采用高压液相色谱法对水溶性维生素（吡哆醇、核黄素、烟酰胺、维生素B12、硫胺素、抗坏血酸、烟酸和叶酸）进行了分离研究。通过使用不同比例的水/甲醇作为洗脱溶剂，并向水/甲醇中添加各种盐、缓冲溶液和PIC试剂，测定了其对各维生素保留时间的影响以及对多种维生素样品的分离效果。每种维生素都能从μBondapak C18柱上令人满意地洗脱下来。结果发现，在几种溶剂系统中，一次分析就能从多种维生素混合物中分离出七种维生素，分析总时间始终小于40分钟。使用μBondapak NH2柱时，除叶酸外的所有维生素都能被洗脱下来，一次分析就能从混合物中分离出六种维生素。μBondapak NH2柱的分析速度更快，所有化合物在15分钟内洗脱完毕，且峰形更尖锐。洗脱顺序与使用μBondapak C18柱时基本相反。
