Patwardhan Sachin, Bloch Sharon, Achilefu Samuel, Culver Joseph
Opt Express. 2005 Apr 4;13(7):2564-77. doi: 10.1364/opex.13.002564.
We present a fast scanning fluorescence optical tomography system for imaging the kinetics of probe distributions through out the whole body of small animals. Configured in a plane parallel geometry, the system scans a source laser using a galvanometer mirror pair (tauswitch~1ms) over flexible source patterns, and detects excitation and emission light using a high frame rate low noise, 5 MHz electron multiplied charge-coupled device (EMCCD) camera. Phantom studies were used to evaluate resolution, linearity, and sensitivity. Time dependent (deltat=2.2 min.) in vivo imaging of mice was performed following injections of a fluorescing probe (indocyanine green). The capability to detect differences in probe delivery route was demonstrated by comparing an intravenous injection, versus an injection into a fat pocket (retro orbital injection). Feasibility of imaging the distribution of tumor-targeted molecular probes was demonstrated by imaging a breast tumor-specific near infrared polypeptide in MDA MB 361 tumor bearing nude mice. A tomography scan, at 24 hour post injection, revealed preferential uptake in the tumor relative to surrounding tissue.
我们展示了一种快速扫描荧光光学断层扫描系统，用于对小动物全身探针分布的动力学进行成像。该系统采用平面平行几何结构，使用一对振镜（切换时间~1ms）在灵活的光源模式下扫描源激光，并使用高帧率、低噪声的5MHz电子倍增电荷耦合器件（EMCCD）相机检测激发光和发射光。通过模型研究评估分辨率、线性度和灵敏度。在注射荧光探针（吲哚菁绿）后，对小鼠进行了时间依赖性（Δt = 2.2分钟）的体内成像。通过比较静脉注射与脂肪囊注射（眶后注射），证明了检测探针输送途径差异的能力。通过对携带MDA MB 361肿瘤的裸鼠体内的乳腺肿瘤特异性近红外多肽进行成像，证明了对肿瘤靶向分子探针分布进行成像的可行性。注射后24小时的断层扫描显示，肿瘤相对于周围组织有优先摄取。
