Nakajima M, Hewitson T D, Mathews D C, Kincaid-Smith P
Department of Nephrology, Royal Melbourne Hospital, Melbourne, Victoria, Australia.
Virchows Arch A Pathol Anat Histopathol. 1991;419(4):267-72. doi: 10.1007/BF01606517.
Intraglomerular extracellular microparticles including so-called virus-like particles and striated membranous structures have been observed in various renal diseases. The presence and localisation of complement components in these extracellular bodies was studied using the protein A-gold electron microscopy method. Ultrastructurally these particles were differentiated into microspherical structures (MSS) and thread-like structures (TS). Both structures showed weak to moderate diffuse labelling with C1s, whilst the intense labelling found with C3d and C9 was confined to individual membrane-like structures of both MSS and TS. Labelling with IgA, IgG, fibrinogen and the complement components C1q, C1r, C3c C4 and C5 showed negative or trace results. There were no differences between the immunolabelling patterns of MSS and TS, nor among different renal diseases in which these structures were found. These findings raise the possibility that formation of so-called virus-like particles such as MSS and TS may be associated with complement activation.
在各种肾脏疾病中均观察到肾小球内细胞外微粒，包括所谓的病毒样颗粒和横纹膜结构。使用蛋白A-金电子显微镜方法研究了这些细胞外小体中补体成分的存在和定位。超微结构上，这些颗粒可分为微球结构（MSS）和线状结构（TS）。两种结构均显示出C1s的弱至中度弥漫性标记，而C3d和C9的强烈标记仅限于MSS和TS的单个膜样结构。IgA、IgG、纤维蛋白原以及补体成分C1q、C1r、C3c、C4和C5的标记显示为阴性或微量结果。MSS和TS的免疫标记模式之间以及发现这些结构的不同肾脏疾病之间均无差异。这些发现增加了一种可能性，即所谓的病毒样颗粒如MSS和TS的形成可能与补体激活有关。
