Cole M J, Chisholm S A, Palmer H M, Wallace L A, Ison C A
Sexually Transmitted Bacteria Reference Laboratory, Health Protection Agency, London NW9 5EQ, UK.
Sex Transm Infect. 2009 Oct;85(6):447-51. doi: 10.1136/sti.2009.036301. Epub 2009 Jun 3.
To examine the molecular epidemiology of syphilis in Scotland.
Ulcer specimens were collected from 85 patients with infectious syphilis. Typing of Treponema pallidum was performed using a method that examines variation in two loci; the number of 60-basepair repeats within the arp gene and sequence variation in the tpr genes.
Patients were predominately white men who have sex with men (MSM). Treponemal DNA was detected in 75 specimens and a total of six subtypes were identified from 58 typeable specimens (77%). The most common subtypes were 14d (44/58, 76%), followed by 14e (7/58, 12%), 14j (3/58, 5%), 14b (2/58, 3%), 14p and 14k (1/58, 2%).
This study shows that subtype 14d is the predominant subtype circulating in Scotland and there is a surprising level of genetic diversity within the Scottish MSM community.
研究苏格兰梅毒的分子流行病学。
从85例感染性梅毒患者中采集溃疡标本。采用检测两个基因座变异的方法对梅毒螺旋体进行分型；arp基因内60个碱基对重复序列的数量以及tpr基因的序列变异。
患者主要为男男性行为者（MSM）。在75份标本中检测到梅毒螺旋体DNA，从58份可分型标本（77%）中总共鉴定出6种亚型。最常见的亚型是14d（44/58，76%），其次是14e（7/58，12%）、14j（3/58，5%）、14b（2/58，3%）、14p和14k（1/58，2%）。
本研究表明，14d亚型是苏格兰流行的主要亚型，并且在苏格兰男男性行为者群体中存在令人惊讶的遗传多样性水平。
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